Application | Comment | Organism |
---|---|---|
synthesis | suitable catalyst for practical syntheses of oligosaccharides | Lachnoclostridium phytofermentans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Lachnoclostridium phytofermentans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
2.8 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
3.3 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
3.4 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
4.5 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
8 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
10 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | proteins are predicted not to possess N-terminal signal peptides | Lachnoclostridium phytofermentans | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
84860 | - |
calculated | Lachnoclostridium phytofermentans |
84940 | - |
calculated | Lachnoclostridium phytofermentans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lachnoclostridium phytofermentans | A9KIW5 | bacterium possesses three GalHexNAcP homologous genes, cphy0577 gene; anaerobic cellulolytic bacterium | - |
Lachnoclostridium phytofermentans | A9KQ75 | bacterium possesses three GalHexNAcP homologous genes, Cphy3030 gene; anaerobic cellulolytic bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Lachnoclostridium phytofermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galacto-N-biose + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
r | |
lacto-N-biose I + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
r | |
lacto-N-biose I + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
additional information | Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal | Lachnoclostridium phytofermentans | ? | - |
? | |
additional information | Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal | Lachnoclostridium phytofermentans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cphy0577 protein | - |
Lachnoclostridium phytofermentans |
cphy3030 protein | - |
Lachnoclostridium phytofermentans |
D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase | - |
Lachnoclostridium phytofermentans |
GalHexNAcP | - |
Lachnoclostridium phytofermentans |
GNB/LNB phosphorylase | - |
Lachnoclostridium phytofermentans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
protein is thermostable up to 30°C | Lachnoclostridium phytofermentans |
45 | - |
protein is thermostable up to 45°C | Lachnoclostridium phytofermentans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
39 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
39 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
40 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
42 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
48 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
49 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
50 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | optimum activity at neutral pH | Lachnoclostridium phytofermentans |