Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.211 extracted from

  • Nakajima, M.; Nishimoto, M.; Kitaoka, M.
    Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase (2009), J. Biol. Chem., 284, 19220-19227.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis suitable catalyst for practical syntheses of oligosaccharides Lachnoclostridium phytofermentans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Lachnoclostridium phytofermentans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
N-acetyl-D-glucosamine
-
Lachnoclostridium phytofermentans
2.8
-
lacto-N-biose I
-
Lachnoclostridium phytofermentans
3.3
-
N-acetyl-D-galactosamine
-
Lachnoclostridium phytofermentans
3.4
-
N-acetyl-D-glucosamine
-
Lachnoclostridium phytofermentans
4.5
-
lacto-N-biose I
-
Lachnoclostridium phytofermentans
8
-
galacto-N-biose
-
Lachnoclostridium phytofermentans
10
-
galacto-N-biose
-
Lachnoclostridium phytofermentans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol proteins are predicted not to possess N-terminal signal peptides Lachnoclostridium phytofermentans 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
SDS-PAGE Lachnoclostridium phytofermentans
84860
-
calculated Lachnoclostridium phytofermentans
84940
-
calculated Lachnoclostridium phytofermentans

Organism

Organism UniProt Comment Textmining
Lachnoclostridium phytofermentans A9KIW5 bacterium possesses three GalHexNAcP homologous genes, cphy0577 gene; anaerobic cellulolytic bacterium
-
Lachnoclostridium phytofermentans A9KQ75 bacterium possesses three GalHexNAcP homologous genes, Cphy3030 gene; anaerobic cellulolytic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galacto-N-biose + phosphate
-
Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
r
lacto-N-biose I + phosphate
-
Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
r
lacto-N-biose I + phosphate
-
Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
additional information Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal Lachnoclostridium phytofermentans ?
-
?
additional information Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal Lachnoclostridium phytofermentans ?
-
?

Synonyms

Synonyms Comment Organism
cphy0577 protein
-
Lachnoclostridium phytofermentans
cphy3030 protein
-
Lachnoclostridium phytofermentans
D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase
-
Lachnoclostridium phytofermentans
GalHexNAcP
-
Lachnoclostridium phytofermentans
GNB/LNB phosphorylase
-
Lachnoclostridium phytofermentans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
protein is thermostable up to 30°C Lachnoclostridium phytofermentans
45
-
protein is thermostable up to 45°C Lachnoclostridium phytofermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34
-
N-acetyl-D-galactosamine
-
Lachnoclostridium phytofermentans
39
-
lacto-N-biose I
-
Lachnoclostridium phytofermentans
39
-
galacto-N-biose
-
Lachnoclostridium phytofermentans
40
-
lacto-N-biose I
-
Lachnoclostridium phytofermentans
42
-
N-acetyl-D-glucosamine
-
Lachnoclostridium phytofermentans
48
-
N-acetyl-D-galactosamine
-
Lachnoclostridium phytofermentans
49
-
N-acetyl-D-glucosamine
-
Lachnoclostridium phytofermentans
50
-
galacto-N-biose
-
Lachnoclostridium phytofermentans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 optimum activity at neutral pH Lachnoclostridium phytofermentans