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Literature summary for 2.4.1.211 extracted from

  • Nishimoto, M.; Kitaoka, M.
    Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk (2007), Biosci. Biotechnol. Biochem., 71, 2101-2104.
    View publication on PubMed
Search for more literature for 2.4.1.211

Application

Application Comment Organism
biotechnology LNBP is cultured with sucrose phosphorylase, UDP-glucose hexose 1-phosphate uridylyltransferase, and UDP-glucose 4-epimerase to produce beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine using sucrose as substrate in a 10l reaction mixture, 500 mmol beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine are produced after 600 h, beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine can be used as bifidus factor in human milk Bifidobacterium bifidum JCM 1254

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bifidobacterium bifidum JCM 1254

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum JCM 1254
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by Ni-NTA spin column chromatography Bifidobacterium bifidum JCM 1254

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 alpha-D-galactose 1-phosphate as substrate Bifidobacterium bifidum JCM 1254

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
 Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
 ?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
 Bifidobacterium bifidum JCM 1254 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
lacto-N-biose phosphorylase
-
 Bifidobacterium bifidum JCM 1254
LNBP
-
 Bifidobacterium bifidum JCM 1254