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Literature summary for 2.4.1.211 extracted from

  • Nakajima, M.; Nihira, T.; Nishimoto, M.; Kitaoka, M.
    Identification of galacto-N-biose phosphorylase from Clostridium perfringens ATCC13124 (2008), Appl. Microbiol. Biotechnol., 78, 465-471.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium perfringens ATCC 13124

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate Bifidobacterium longum subsp. Longum
0.52
-
phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate Clostridium perfringens ATCC 13124
0.58
-
phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine as substrate Bifidobacterium longum subsp. Longum
0.66
-
phosphate beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine as substrate Clostridium perfringens ATCC 13124
1.7
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine sequential bi-bi mechanism Clostridium perfringens ATCC 13124
2.1
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
Bifidobacterium longum subsp. Longum
5.6
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine
-
Bifidobacterium longum subsp. Longum
21
-
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine
-
Clostridium perfringens ATCC 13124

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
SDS-PAGE Clostridium perfringens ATCC 13124

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. Longum
-
-
-
Bifidobacterium longum subsp. Longum JCM 1217
-
-
-
Clostridium perfringens ATCC 13124
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by Ni-nitrilotriacetic acid agarose column chromatography Clostridium perfringens ATCC 13124

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate no activity with D-glucose, D-glucosamine, D-fructose, 2-deoxy-D-glucose, D-mannose, maltose, and sucrose as substrates Clostridium perfringens ATCC 13124 alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
Bifidobacterium longum subsp. Longum alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-galactosmine + phosphate
-
Bifidobacterium longum subsp. Longum JCM 1217 alpha-D-galactose 1-phosphate + N-acetyl-galactosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Clostridium perfringens ATCC 13124 alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Bifidobacterium longum subsp. Longum alpha-D-galactose 1-phosphate + N-acetyl-glucosamine
-
?
beta-D-galactopyranosyl-1,3-N-acetyl-D-glucosamine + phosphate
-
Bifidobacterium longum subsp. Longum JCM 1217 alpha-D-galactose 1-phosphate + N-acetyl-glucosamine
-
?

Synonyms

Synonyms Comment Organism
galacto-N-biose phosphorylase
-
Bifidobacterium longum subsp. Longum
galacto-N-biose phosphorylase
-
Clostridium perfringens ATCC 13124
LNBP
-
Bifidobacterium longum subsp. Longum
LNBP
-
Clostridium perfringens ATCC 13124

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Clostridium perfringens ATCC 13124

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 in 100 mM buffer containing 20 micromolar DTT Clostridium perfringens ATCC 13124

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Clostridium perfringens ATCC 13124

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5.5 in presence of sodium acetate Clostridium perfringens ATCC 13124
5.5 6.5 in presence of MES-NaOH Clostridium perfringens ATCC 13124
6.5 7.5 in presence of Mops-NaOH Clostridium perfringens ATCC 13124
7.5 9 in presence of Tris-HCl Clostridium perfringens ATCC 13124

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 in presence of sodium acetate Clostridium perfringens ATCC 13124
6 7 in presence of MES-NaOH Clostridium perfringens ATCC 13124
6.5 7.5 in presence of Mops-NaOH Clostridium perfringens ATCC 13124
7.5 9 in presence of Tris-HCl Clostridium perfringens ATCC 13124