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Literature summary for 2.4.1.210 extracted from
- Functional characterization and reclassification of an enzyme previously proposed to be a limonoid UDP-glucosyltransferase (2020), J. Sci. Food. Agric., 100, 4870-4878 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | major problem in the orange industry is delayed bitterness, which is caused by limonin, a bitter compound developing from its non-bitter precursor limonoate A-ring lactone (LARL) during and after extraction of orange juice. The glucosidation of LARL by limonoid UDP-glucosyltransferase (LGT) to form non-bitter glycosyl-limonin during orange maturation has been demonstrated as a natural way to debitter by preventing the formation of limonin. Enzyme LGT activity can be used to develop biotechnology-based approaches for producing orange juice from varietals that traditionally have a delayed bitterness | Citrus unshiu |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the MBP-tagged enzyme (MBP-cuGT) in Escherichia coli strain BLR(DE3). MBP-cuGT does not exhibit any detectable effect on limonin degradation when Navel orange juice is used as the substrate. MBP-cuGT is unable to biotransform either limonoate A-ring lactone (LARL) or limonoate as purified substrates. But MBP-cuGT displays a broad activity spectrum towards flavonoids, confirming that the enzyme produced is active under the conditions evaluated in vitro, expression method evaluation, overview | Citrus unshiu |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Citrus unshiu |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + limonin | Citrus unshiu | - |
glucosyl-limonin + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus unshiu | Q9MB73 | i.e. Citrus nobilis var. unshiu | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant MBP-tagged enzyme from Escherichia coli strain BLR(DE3) by cobalt affinity chromatography | Citrus unshiu |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + limonin | - |
Citrus unshiu | glucosyl-limonin + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 103000, recombinant MBP-tagged enzyme, SDS-PAGE | Citrus unshiu |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cuGT | - |
Citrus unshiu |
| LGT | - |
Citrus unshiu |
| limonoid UDP-glucosyltransferase | - |
Citrus unshiu |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Citrus unshiu |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | assay at | Citrus unshiu |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme homology structure modeling | Citrus unshiu |
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Release 2023.1 (January 2023)
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