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Literature summary for 2.4.1.210 extracted from
- Cloning and characterization of limonoid glucosyltransferase from Kinnow mandarin (Citrus reticulata Blanco) (2018), Food Technol. Biotechnol., 56, 228-237 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | overexpression of the LGT gene can reduce the delayed bitterness problem in citrus juice | Citrus reticulata |
| medicine | overexpression of the LGT gene can enhance the accumulation of specific glucosides that have anticancer effects. Citrus limoxadnoids, specifically limonoid glucosides, which are the prodxaduct of glucosyltransferase enzyme, are water-soluble and have good bioavailability and non-toxic effects in animals and huxadmans. Thus, they can easily be used as nutraceuticals and health fortifiers in many functional foods | Citrus reticulata |
| nutrition | overexpression of the LGT gene can enhance the accumulation of specific glucosides that have anticancer effects. Citrus limoxadnoids, specifically limonoid glucosides, which are the prodxaduct of glucosyltransferase enzyme, are water-soluble and have good bioavailability and non-toxic effects in animals and huxadmans. Thus, they can easily be used as nutraceuticals and health fortifiers in many functional foods | Citrus reticulata |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CrLGT cloning of full-length gene from a cDNA library, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, RT-PCR enzyme expression analyxadsis | Citrus reticulata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + ichangin | Citrus reticulata | - |
glucosyl-ichangin + UDP | - |
? |  |
| UDP-glucose + limonin | Citrus reticulata | - |
limonin 17-beta-D-glucopyranoside + UDP | - |
? | |
| UDP-glucose + nomilin | Citrus reticulata | - |
glucosyl-nomilin + UDP | - |
? | |
| UDP-glucose + obacunone | Citrus reticulata | - |
glucosyl-obacunone + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus reticulata | A0A1I7P0P5 | Kinnow mandarin, collected from Fruit Research Farm, Punjab Agricultural University, Ludhiana, India, during the seasons 2014-2016 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| albedo | inner part of fruit peel | Citrus reticulata | - |
| flavedo | outer part of fruit peel | Citrus reticulata | - |
| fruit | flavedo, albedo, an sac covering | Citrus reticulata | - |
| leaf | young | Citrus reticulata | - |
| additional information | transcript expression of CrLGT in vegetative and fruit tissues of Kinnow mandarin, evaluated in different tissues such as young leaf, flavedo, albedo, sac covering and seed of Kinnow mandarin during early (90 days after flowering (DAF)), mid (150-210 DAF) and late (240 DAF) fruit developmental stages using semi-quantitative method, shows the highest expression in flavedo | Citrus reticulata | - |
| seed | - |
Citrus reticulata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + ichangin | - |
Citrus reticulata | glucosyl-ichangin + UDP | - |
? | |
| UDP-glucose + limonin | - |
Citrus reticulata | limonin 17-beta-D-glucopyranoside + UDP | - |
? | |
| UDP-glucose + nomilin | - |
Citrus reticulata | glucosyl-nomilin + UDP | - |
? | |
| UDP-glucose + obacunone | - |
Citrus reticulata | glucosyl-obacunone + UDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrLGT | - |
Citrus reticulata |
| LGT | - |
Citrus reticulata |
| limonoid UDP glucosyltransferase | - |
Citrus reticulata |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the superfamily PLN03004 (UDP-glycosyltransferase). Citrus reticulata limonoid glucosyltransferase (CrLGT) occupies a position on an independent branch in the largest subgroup and is phylogenetically different from those in other mandarin species like Citrus unshiu, showing its uniqueness in several features | Citrus reticulata |
| malfunction | overexpression of the LGT gene can reduce the delayed bitterness problem in citrus juice and enhance the accumulation of specific glucosides that have anticancer effects | Citrus reticulata |
| metabolism | enzyme LGT is important in the limonoid metabolism pathway in Citrus plants. Limonoid glucosyltransfease (LGT) is the only single regulatory enzyme that is able to convert all limonoid aglycones such as nomilin, obacunone, and ichangin present in Citrus spp. to their respective glucosides, in addition to limonoate A-ring lactone (LARL) to limonin glucopyranoside. During juicing process bitter limonin is immediately produced from LARL precursor by limonin D-ring lactone hydrolase (LLH) regulatory enzyme, especially in early to mid-stage developed Citrus fruits | Citrus reticulata |
| physiological function | limonoid glucosyltransferase (LGT) is a natural debittering enzyme, because the delayed bitterness problem is caused mainly by limonoid aglycones such as limonin. THe product of LGT reaction, limonin 17-beta-D-glucopyranoside, in non-bitter and has anticancer potential in animals including humans | Citrus reticulata |
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