Application | Comment | Organism |
---|---|---|
medicine | pharmacological application of limonoid GTase gene | Citrus sinensis |
nutrition | - |
Citrus sinensis |
Cloned (Comment) | Organism |
---|---|
cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome | Citrus unshiu |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Citrus sinensis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57500 | - |
predicted from amino acid sequence | Citrus unshiu |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
limonin + UDP-glucose | Citrus sinensis | - |
glucosyl-limonin + UDP | - |
? | |
limonin + UDP-glucose | Citrus unshiu | - |
glucosyl-limonin + UDP | - |
? | |
nomilin + UDP-glucose | Citrus sinensis | - |
UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
nomilin + UDP-glucose | Citrus unshiu | - |
UDP + nomilin 17-beta-D-glucopyranoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | - |
sweet navel orange Osb. cv. Washington | - |
Citrus unshiu | - |
satsuma mandarin Marc. cv. Miyagawa-wase | - |
Purification (Comment) | Organism |
---|---|
- |
Citrus sinensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + limonin + H2O = glucosyl-limonin + UDP | the enzyme purified from navel orange albedo tissue also acts on the related tetranortriterpenoid nomilin | Citrus sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
albedo | inner spongy part of rind | Citrus sinensis | - |
Storage Stability | Organism |
---|---|
-80°C, 10 mM Mes-KOH, pH 7 allows frozen storage with full recovery of activity | Citrus sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
limonin + UDP-glucose | - |
Citrus sinensis | glucosyl-limonin + UDP | - |
? | |
limonin + UDP-glucose | - |
Citrus unshiu | glucosyl-limonin + UDP | - |
? | |
nomilin + UDP-glucose | - |
Citrus sinensis | UDP + nomilin 17-beta-D-glucopyranoside | - |
? | |
nomilin + UDP-glucose | - |
Citrus unshiu | UDP + nomilin 17-beta-D-glucopyranoside | - |
? |