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Literature summary for 2.4.1.21 extracted from

  • Cuesta-Seijo, J.A.; Nielsen, M.M.; Marri, L.; Tanaka, H.; Beeren, S.R.; Palcic, M.M.
    Structure of starch synthase I from barley: insight into regulatory mechanisms of starch synthase activity (2013), Acta Crystallogr. Sect. D, 69, 1013-1025.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner(DE3) cells Hordeum vulgare

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM sodium cacodylate buffer pH 6.5, 17% (w/v)v PEG 8K, 0.2 M ammonium sulfate Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
C126S the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
C506S the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare
F538A the mutant shows strongly reduced activity compared to the wild type enzyme Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Hordeum vulgare
-
-

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q9M5A3
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography and gel filtration Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + glycogen
-
Hordeum vulgare ADP + ?
-
?
ADP-alpha-D-glucose + maltopentaose
-
Hordeum vulgare ADP + maltohexaose
-
?
ADP-alpha-D-glucose + maltotriose
-
Hordeum vulgare ADP + maltotetraose
-
?
ADP-alpha-D-glucose + soluble starch
-
Hordeum vulgare ADP + ?
-
?

Synonyms

Synonyms Comment Organism
ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase
-
Hordeum vulgare
SSI
-
Hordeum vulgare
starch synthase I
-
Hordeum vulgare