Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner(DE3) cells | Hordeum vulgare |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM sodium cacodylate buffer pH 6.5, 17% (w/v)v PEG 8K, 0.2 M ammonium sulfate | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
C126S | the mutant shows strongly reduced activity compared to the wild type enzyme | Hordeum vulgare |
C506S | the mutant shows strongly reduced activity compared to the wild type enzyme | Hordeum vulgare |
F538A | the mutant shows strongly reduced activity compared to the wild type enzyme | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Hordeum vulgare | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q9M5A3 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity column chromatography and gel filtration | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + glycogen | - |
Hordeum vulgare | ADP + ? | - |
? | |
ADP-alpha-D-glucose + maltopentaose | - |
Hordeum vulgare | ADP + maltohexaose | - |
? | |
ADP-alpha-D-glucose + maltotriose | - |
Hordeum vulgare | ADP + maltotetraose | - |
? | |
ADP-alpha-D-glucose + soluble starch | - |
Hordeum vulgare | ADP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase | - |
Hordeum vulgare |
SSI | - |
Hordeum vulgare |
starch synthase I | - |
Hordeum vulgare |