Protein Variants | Comment | Organism |
---|---|---|
W366A | mutation in starch-binding domains, 3fold decrease in affinity to starch | Arabidopsis thaliana |
W366A/Y394A | mutation in starch-binding domains, significant decrease in affinity to starch | Arabidopsis thaliana |
Y394A | mutation in starch-binding domains, 2fold decrease in affinity to starch | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
ADP-glucose | catalytic domain | Arabidopsis thaliana | |
0.59 | - |
ADP-glucose | catalytic domain co-expressed with starch-binding domain 3 and large part of starch-binding domain 2 | Arabidopsis thaliana | |
0.62 | - |
ADP-glucose | catalytic domain co-expressed with starch-binding domains 23 | Arabidopsis thaliana | |
0.81 | - |
ADP-glucose | catalytic domain co-expressed with starch-binding domain 2 and part of starch-binding domain 3 | Arabidopsis thaliana | |
0.95 | - |
ADP-glucose | catalytic domain co-expressed with starch-binding domains 123 | Arabidopsis thaliana | |
1.68 | - |
ADP-glucose | truncated protein lacking starch binding domain 1 and large part of starch binding domain 2 | Arabidopsis thaliana | |
1.74 | - |
ADP-glucose | truncated protein lacking starch-binding domains 12 | Arabidopsis thaliana | |
1.77 | - |
ADP-glucose | truncated protein lacking starch binding domain 1 and medium part of starch binding domain 2 | Arabidopsis thaliana | |
2.39 | - |
ADP-glucose | truncated protein lacking starch binding domain 1 and small part of starch binding domain 2 | Arabidopsis thaliana | |
2.56 | - |
ADP-glucose | truncated protein lacking starch-binding domain 1 | Arabidopsis thaliana | |
4.08 | - |
ADP-glucose | wild-type | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
starch synthase III | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + glycogen | - |
Arabidopsis thaliana | ADP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme contains an N-terminal region, including three in-tandem starch-binding domains, followed by a C-terminal catalytic domain. The D(316-344) and D(495-535) regions in the D2 and D3 domains, respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. Residues W366 and Y394 in the D2 domain are important in starch binding. Residue W366 is key to the apparent affinity for the polysaccharide substrate of starch synthase III | Arabidopsis thaliana |