Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 500 mM, 4fold activation of unprimed starch synthesis | Zea mays | |
citrate | 500 mM, 20fold activation of unprimed starch synthesis | Zea mays | |
succinate | 500 mM, 6fold activation of unprimed starch synthesis | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | amylopectin: 0.16 mg/ml, rabbit liver glycogen: 1.16 mg/ml | Zea mays | |
0.033 | - |
ADPglucose | - |
Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + alpha-1,4-polyglucan | Zea mays | involved in starch biosynthesis | ADP + alpha-1,4-polyglucan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, aminobutyl-Sepharose, ADP-hexanolamine-Sepharose, aminopropyl-Sepharose | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
unprimed reaction, synthase I | Zea mays |
2.3 | - |
primed reaction, synthase I | Zea mays |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris/acetate buffer, pH 7.5, 2.5 mM DTT, 10 mM EDTA, 5% w/v sucrose | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + alpha-1,4-polyglucan | citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen | Zea mays | ADP + alpha-1,4-polyglucan | - |
? | |
ADP-glucose + alpha-1,4-polyglucan | involved in starch biosynthesis | Zea mays | ADP + alpha-1,4-polyglucan | - |
? |