Cloned (Comment) | Organism |
---|---|
gene XTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, constitutive recombinant expression in Nicotiana tabacum plants via transfection with Agrobacterium tumefaciens strain AGL0 | Populus tremula |
gene XTH1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis | Populus tremula |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of transgenic Nicotiana tabacum plants with constitutive expression of the PnXTH1 gene (the orthologue of PtrXTH1 from the black poplar, Populus nigra). Transgenic tobacco plants are characterized by an increase in leaf size and fresh and dry weight of the above ground part under normal growth conditions. When grown under conditions of salinization and drought, transgenic plants show increased stress resistance due to the maintenance of cell expansion in roots and stems at a higher level and the ability to more effectively retain water in leaves compared with wild-type plants. Molecular and morphological analysis of transgenic tobacco plants, phenotype, overview. The constitutive expression of PnXTH1 promotes an increase in the fresh and dry weight of the aboveground part of the plants mainly due to stimulation of leaf growth. Moreover, the degree of morphological changes in transgenic plants correlate with the expression level of PnXTH1. The root length increases substantially with the action of salt in all transgenic plants | Populus tremula |
Organism | UniProt | Comment | Textmining |
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Populus tremula | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highest level of transcripts of PtrXTH1 is detected in young, intensively growing leaves | Populus tremula | - |
Synonyms | Comment | Organism |
---|---|---|
PtnXTH1 | - |
Populus tremula |
PtrXTH1 | - |
Populus tremula |
xyloglucan endo-transglycosylase | - |
Populus tremula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Populus tremula |
Organism | Comment | Expression |
---|---|---|
Populus tremula | in young aspen leaves, the expression of PtrXTH1 is induced by cytokinins, auxins, and brassinosteroids. The content of PtrXTH1 transcripts increases under the constitutive expression of the PnARGOS-LIKE (UniProt ID I6SWL6) gene. Participation of the PnARGOS-LIKE gene in the regulation of PtrXTH1 gene expression | up |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of the nucleotide sequences of the open reading frames of the XTHs genes of Arabidopsis thaliana and poplar PnXTH1 shows that this gene is the closest to genes AtXTH5 (endoxyloglucan transferase A4, EXGT-A4) and AtXTH4 (endoxyloglucan transferase A1, EXGT-A1). Identification and analysis of the cis-regulatory elements of the putative promoter region of PnXTH1 gene orthologue from Populus trichocarpa PtXTH1 | Populus tremula |
malfunction | the constitutive expression of PnXTH1 in transgenic Nicotiana tabacum promotes an increase in the fresh and dry weight of the aboveground part of the plants mainly due to stimulation of leaf growth. Moreover, the degree of morphological changes in transgenic plants correlate with the expression level of PnXTH1. The root length increases substantially with the action of salt in all transgenic plants | Populus tremula |
physiological function | the enzyme is involved in regulation of plant growth and stress resistance | Populus tremula |