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Literature summary for 2.4.1.207 extracted from

  • Witasari, L.D.; Huang, F.C.; Hoffmann, T.; Rozhon, W.; Fry, S.C.; Schwab, W.
    Higher expression of the strawberry xyloglucan endotransglucosylase/hydrolase genes FvXTH9 and FvXTH6 accelerates fruit ripening (2019), Plant J., 100, 1237-1253 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH6 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH9, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells Fragaria vesca
phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum via Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells Fragaria vesca

Protein Variants

Protein Variants Comment Organism
additional information Fragaria x ananassa cv. Elsanta fruits infiltrated with FvXTH9 and FvXTH6 ripen faster and show decreased firmness compared with the empty vector control pBI121 Fragaria vesca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Fragaria vesca

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information prediction of subcellular localization suggests a localization to the secretory pathway Fragaria vesca
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Organism

Organism UniProt Comment Textmining
Fragaria vesca
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cv. Hawaii 4
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography Fragaria vesca

Source Tissue

Source Tissue Comment Organism Textmining
flower high expression level of FvXTH6 Fragaria vesca
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flower high expression level of FvXTH9 Fragaria vesca
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fruit FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages Fragaria vesca
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fruit the expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages Fragaria vesca
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leaf FvXTH6 is expressed at a relatively high level in young leaf tissue but at a low level in old leaf tissue Fragaria vesca
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additional information to confirm the transcriptome data, the expression pattern of FvXTH6 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. The expression pattern of FvXTH6 during fruit development shows the highest level in fully developed green fruit and with a clear decline in later stages. The absolute expression level of FvXTH6 in fruit is much lower than that of FvXTH9. FvXTH6 is also highly expressed in flowers. FvXTH6 is expressed at relatively high level in young leaf tissue but at low level in old leaf tissue Fragaria vesca
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additional information to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles Fragaria vesca
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE, EC 2.4.1.B52), cellulose:xyloglucan endotransglucosylase (CXE, EC 2.4.1.B52), and xyloglucan endotransglucosylase (XET) activities Fragaria vesca ?
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tamarind xyloglucan + XLLGol XET activity Fragaria vesca ?
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?
tamarind xyloglucan + XXFGol XET activity Fragaria vesca ?
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?
tamarind xyloglucan + XXGol XET activity Fragaria vesca ?
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?
tamarind xyloglucan + XXLGol XET activity Fragaria vesca ?
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?
tamarind xyloglucan + XXXGol XET activity Fragaria vesca ?
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?

Subunits

Subunits Comment Organism
? x * 33200, about, sequence calculation Fragaria vesca

Synonyms

Synonyms Comment Organism
FvXTH6
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Fragaria vesca
FvXTH9
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Fragaria vesca
More see also EC 2.4.1.B52 Fragaria vesca
XET
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Fragaria vesca
XTH6
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Fragaria vesca
XTH9
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Fragaria vesca
xyloglucan endotransglucosylase
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Fragaria vesca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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assay at Fragaria vesca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Fragaria vesca

pI Value

Organism Comment pI Value Maximum pI Value
Fragaria vesca sequence calculation
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5.46

General Information

General Information Comment Organism
evolution XTHs are classified together with the lichenases, which hydrolyse mixed-linkage (1->3,1->4)-beta-D-glucans (MLG), in glycoside hydrolase family 16 Fragaria vesca
metabolism enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE) Fragaria vesca
metabolism enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE). XTHs (xyloglucan endotransglucosylases/hydrolases) can catalyse the endolytic cleavage of xyloglucan polymers and the rejoining of the newly generated reducing ends to other xyloglucan molecules, which is referred to as xyloglucan endotransglucosylase (XET) activity. In addition, XTHs can also show xyloglucan endohydrolase (XEH) activity, where water is used as an acceptor, and thus the xyloglucan molecule is hydrolysed Fragaria vesca
physiological function FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid Fragaria vesca
physiological function FvXTH9 and also FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid Fragaria vesca