Literature summary for 2.4.1.207 extracted from

  • Yan, J.; Huang, Y.; He, H.; Han, T.; Di, P.; Sechet, J.; Fang, L.; Liang, Y.; Scheller, H.; Mortimer, J.; Ni, L.; Jiang, M.; Hou, X.; Zhang, A.; Zhang, J.
    Xyloglucan endotransglucosylase-hydrolase30 negatively affects salt tolerance in Arabidopsis (2019), J. Exp. Bot., 70, 5495-5506 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Commentary) Organism
gene XTH30, recombinant expression of GFP- or YFP-tagged isozyme XTH30 in Arabidopsis thaliana and heterologous expression of wild-type and mutant enzymes in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation, real-time PCR enzyme expression analysis. Expression of a XTH30 promoter::GUS construct in Arabidopsis thaliana Arabidopsis thaliana

Engineering

Protein Variants Commentary Organism
additional information generation of loss-of-function of XTH30 mutants, xth30 mutants xth30-1 and xth30-2, phenotype, overview. Homozygous xth30 mutants are identified by T-DNA insertion-based PCR. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment Arabidopsis thaliana

Localization

Localization Commentary Organism GeneOntology No. Textmining
plasma membrane
-
Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Commentary Organism Structure
NaCl induces an increase in XTH30 expression and xyloglucan (XyG)-derived oligosaccharides (XLFG) in the wild-type Arabidopsis thaliana

Organism

Organism UniProt Commentary Textmining
Arabidopsis thaliana Q38908
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-
Arabidopsis thaliana Col-0 Q38908
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-

Source Tissue

Source Tissue Commentary Organism Textmining
root
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
additional information XTH30 is highly expressed in the root, flower, stem, and etiolated hypocotyl. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves, real-time PCR enzyme expression analysis, expression pattern, overview Arabidopsis thaliana
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flower
-
Arabidopsis thaliana
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hypocotyl etiolated Arabidopsis thaliana
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cauline leaf
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Arabidopsis thaliana
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rosette leaf
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Arabidopsis thaliana
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Synonyms

Synonyms Commentary Organism
xyloglucan endotransglucosylase-hydrolase30
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Arabidopsis thaliana
XTH30
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Arabidopsis thaliana

Cloned(Commentary) (protein specific)

Commentary Organism
gene XTH30, recombinant expression of GFP- or YFP-tagged isozyme XTH30 in Arabidopsis thaliana and heterologous expression of wild-type and mutant enzymes in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation, real-time PCR enzyme expression analysis. Expression of a XTH30 promoter::GUS construct in Arabidopsis thaliana Arabidopsis thaliana

Engineering (protein specific)

Protein Variants Commentary Organism
additional information generation of loss-of-function of XTH30 mutants, xth30 mutants xth30-1 and xth30-2, phenotype, overview. Homozygous xth30 mutants are identified by T-DNA insertion-based PCR. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment Arabidopsis thaliana

Localization (protein specific)

Localization Commentary Organism GeneOntology No. Textmining
plasma membrane
-
Arabidopsis thaliana
-
-

Metals/Ions (protein specific)

Metals/Ions Commentary Organism Structure
NaCl induces an increase in XTH30 expression and xyloglucan (XyG)-derived oligosaccharides (XLFG) in the wild-type Arabidopsis thaliana

Source Tissue (protein specific)

Source Tissue Commentary Organism Textmining
root
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
additional information XTH30 is highly expressed in the root, flower, stem, and etiolated hypocotyl. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves, real-time PCR enzyme expression analysis, expression pattern, overview Arabidopsis thaliana
-
flower
-
Arabidopsis thaliana
-
hypocotyl etiolated Arabidopsis thaliana
-
cauline leaf
-
Arabidopsis thaliana
-
rosette leaf
-
Arabidopsis thaliana
-

Expression

Organism Commentary Expression
Arabidopsis thaliana expression of XTH30, encoding xyloglucan endotransglucosylase-hydrolase 30, is strongly upregulated in response to salt stress in Arabidopsis thaliana. Salt stress decreases the relative level of XXFG and XXFG+Ac, but increases the level of XXXG, XLFG, and XLFG+Ac in the wild-type. More importantly, the increase of XLFG caused by salt stress in the wild-type is partly blocked in xth30 mutants. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves up
Arabidopsis thaliana no growth phenotype difference is observed between xth30 mutants and wild-type under heavy metal stresses (CdCl2 and ZnSO4) or the plant hormone abscisic acid (ABA) treatment additional information

General Information

General Information Commentary Organism
physiological function xyloglucan (XyG) is an important hemicellulose polymer of the primary cell wall in dicotyledons and non-commelinid monocotyledons. XyG plays a vital role in loosening or stiffening the cell wall by binding to cellulose microfibrils with hydrogen bonds during cell elongation. XyG chains can be cleaved or rejoined by xyloglucan endotransglucosylase-hydrolase (XTH). Alteration to the cell wall is one strategy that helps plants adapt to salt stress. Isozyme xyloglucan endotransglucosylase-hydrolase 30 (XTH30) negatively affects salt tolerance in Arabidopsis thaliana. XTH30 modulates XyG side chains, alters abundance of XLFG, cellulose synthesis, and cortical microtubule stability, and negatively affects salt tolerance. And XTH30 aggravates depolymerization of cortical microtubules under salt stress Arabidopsis thaliana
malfunction loss-of-function of XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. Loss-of-function of XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Lower Na+ accumulation in shoot and lower H2O2 content are found in xth30 mutants in response to salt stress. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment. XTH30 affects the xyloglucan oligosaccharide composition during salt stress Arabidopsis thaliana

General Information (protein specific)

General Information Commentary Organism
physiological function xyloglucan (XyG) is an important hemicellulose polymer of the primary cell wall in dicotyledons and non-commelinid monocotyledons. XyG plays a vital role in loosening or stiffening the cell wall by binding to cellulose microfibrils with hydrogen bonds during cell elongation. XyG chains can be cleaved or rejoined by xyloglucan endotransglucosylase-hydrolase (XTH). Alteration to the cell wall is one strategy that helps plants adapt to salt stress. Isozyme xyloglucan endotransglucosylase-hydrolase 30 (XTH30) negatively affects salt tolerance in Arabidopsis thaliana. XTH30 modulates XyG side chains, alters abundance of XLFG, cellulose synthesis, and cortical microtubule stability, and negatively affects salt tolerance. And XTH30 aggravates depolymerization of cortical microtubules under salt stress Arabidopsis thaliana
malfunction loss-of-function of XTH30 leads to increased salt tolerance and overexpression of XTH30 results in salt hypersensitivity. Loss-of-function of XTH30 slows down the decrease of crystalline cellulose content and the depolymerization of microtubules caused by salt stress. Lower Na+ accumulation in shoot and lower H2O2 content are found in xth30 mutants in response to salt stress. The xth30 mutants wilt less and show a much higher survival rate than the wild-type under 125 mM NaCl treatment. XTH30 affects the xyloglucan oligosaccharide composition during salt stress Arabidopsis thaliana

Expression (protein specific)

Organism Commentary Expression
Arabidopsis thaliana expression of XTH30, encoding xyloglucan endotransglucosylase-hydrolase 30, is strongly upregulated in response to salt stress in Arabidopsis thaliana. Salt stress decreases the relative level of XXFG and XXFG+Ac, but increases the level of XXXG, XLFG, and XLFG+Ac in the wild-type. More importantly, the increase of XLFG caused by salt stress in the wild-type is partly blocked in xth30 mutants. XTH30 accumulates at high levels in root, stem, and flower but at low levels in rosette leaves and cauline leaves up
Arabidopsis thaliana no growth phenotype difference is observed between xth30 mutants and wild-type under heavy metal stresses (CdCl2 and ZnSO4) or the plant hormone abscisic acid (ABA) treatment additional information