Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.207 extracted from

  • Jiang, Y.; Li, Y.; Lu, C.; Tang, Y.; Jiang, X.; Gai, Y.
    Isolation and characterization of Populus xyloglucan endotransglycosylase/hydrolase (XTH) involved in osmotic stress responses (2020), Int. J. Biol. Macromol., 155, 1277-1287 .
    View publication on PubMed
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH27 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants Populus tomentosa
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH34 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants Populus tomentosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Populus tomentosa 5618
-

Organism

Organism UniProt Comment Textmining
Populus tomentosa A0A2L0E8U4
-
-
Populus tomentosa A0A2L0E8V5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of isozymes PtoXTH27 and PtoXTH34, PtoXTH34 has a higher affinity for XXXG. The receptor substrate of XTH in plants may be XXXG, or xyloglucan oligosaccharides with different chain lengths or structures, such as XLLG and XXXGXXXG. The different affinities of PtoXTH34 and PtoXTH27 for XXXG may be due to the diversity of receptor substrates in plants. Additionally, PtoXTH27 may have other receptor substrates with higher affinity Populus tomentosa ?
-
-

Subunits

Subunits Comment Organism
? x * 33930, PtoXTH27, sequence calculation Populus tomentosa
? x * 34180, PtoXTH34, sequence calculation Populus tomentosa
More comparison with 10 other XTH isozymes from Populus tomentosa, overview Populus tomentosa

Synonyms

Synonyms Comment Organism
PtoXTH27
-
 Populus tomentosa
PtoXTH34
-
 Populus tomentosa
XET
-
 Populus tomentosa
XTH
-
 Populus tomentosa
xyloglucan endotransglucosylase
-
 Populus tomentosa
xyloglucan endotransglycosylase/hydrolase
-
 Populus tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Populus tomentosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Populus tomentosa
6
-
-
 Populus tomentosa

pI Value

Organism Comment pI Value Maximum pI Value
Populus tomentosa comparison with 10 other XTH genes from Populus tomentosa, overview
-
 additional information
Populus tomentosa PtoXTH27, sequence calculation
-
 8.19
Populus tomentosa PtoXTH34, sequence calculation
-
 8.47

General Information

General Information Comment Organism
evolution xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes. 11 members of the XTH gene family are cloned from Populus tomentosa. A bioinformatics analysis reveals that the 11 PtoXTHs can be classified into three groups. Biochemical analyses of purified PtoXTHs demonstrate that only isozymes PtoXTH27 and PtoXTH34 have detectable xyloglucan endotransglucosylase (XET) activity, while the others do not exhibit XET or XEH activity. PtoXTH34 has a higher affinity for the receptor substrate implying that PtoXTH34 and PtoXTH27 in plants have different substrate structure specificity. Members of the XTH gene family may have one or two enzymatic activities: one is xyloglucan endohydrolase (XEH) activity, which specifically hydrolyzes xyloglucan beta-1,4 glycosidic bonds, and the other is xyloglucan endotransglucosylase (XET) activity, which refers to the transfer of xyloglucan fragments between xyloglucan molecules Populus tomentosa
additional information structural modeling and analysis of PtoXTH proteins Populus tomentosa
physiological function Populus xyloglucan endotransglycosylase/hydrolase (XTH) plays an important role in the structure and function of plant cell walls and is involved in osmotic stress responses Populus tomentosa