Cloned (Comment) | Organism |
---|---|
gene XTH8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression in onion epidermal cells at the cell wall, recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum | Diospyros kaki |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum, DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants | Diospyros kaki |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | transient expression assays in onion epidermal cells indicate direct localization of DkXTH8 to the cell wall via its signal peptide | Diospyros kaki | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diospyros kaki | A0A067YMX8 | cv. Fupingjianshi, i.e. Diospyros chinensis | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
calyx | very low expression level | Diospyros kaki | - |
flower | very low expression level | Diospyros kaki | - |
fruit | ripe fruits, mainly | Diospyros kaki | - |
leaf | very low expression level | Diospyros kaki | - |
additional information | transcriptional profiling reveals that DkXTH8 peaks during dramatic fruit softening. Very lo expression levels in flowers, calyces, leaves and stems, respectively | Diospyros kaki | - |
stem | very low expression level | Diospyros kaki | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | when expressed in vitro, the recombinant DkXTH8 protein exhibits strict xyloglucan endotransglycosylase activity (XET, EC 2.4.1.207), whereas no xyloglucan endohydrolase activity (XEH, EC 3.2.1.151) is observed | Diospyros kaki | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DkXTH8 | - |
Diospyros kaki |
XET | - |
Diospyros kaki |
xyloglucan endotransglucosylase | - |
Diospyros kaki |
xyloglucan endotransglucosylase/hydrolase | - |
Diospyros kaki |
Organism | Comment | Expression |
---|---|---|
Diospyros kaki | expression of DkXTH8 is suppressed by gibberellic acid and 1-methylcyclopropene during storage at room temperature | down |
Diospyros kaki | expression of DkXTH8 is stimulated by propylene and abscisic acid during storage at room temperature | up |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of DkXTH8 results in increased leaf senescence coupled with higher electrolyte leakage in Arabidopsis and faster fruit ripening and softening rates in tomato. Transgenic plants overexpressing DkXTH8 display more irregular and twisted cells due to cell wall restructuring, resulting in wider interstitial spaces with less compact cells. DkXTH8 expression causes cells to be easily destroyed, increases membrane permeability and cell peroxidation, and accelerates leaf senescence and fruit softening in transgenic plants | Diospyros kaki |
physiological function | fruit softening is mainly associated with cell wall structural modifications, and members of the xyloglucan endotransglucosylase/hydrolase (XTH) family are key enzymes involved in cleaving and rejoining xyloglucan in the cell wall. Isozyme DkXTH8, a xyloglucan endotransglucosylase/hydrolase in persimmon, alters cell wall structure and promotes leaf senescence and fruit postharvest softening | Diospyros kaki |