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Literature summary for 2.4.1.207 extracted from
- Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening (2015), PLoS ONE, 10, e0123668 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene XTH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Diospyros kaki |
| gene XTH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 | Diospyros kaki |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | the coding protein of DkXTH1 is targeted to the cell wall by its N-terminal signal peptide | Diospyros kaki | 5618 | - |
| cell wall | the coding protein of DkXTH2 is targeted to the cell wall by its N-terminal signal peptide | Diospyros kaki | 5618 | - |
| additional information | the enzyme contains a signal peptide | Diospyros kaki | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan | Diospyros kaki | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diospyros kaki | G5DAC6 | cv. Fupingjianshi, i.e. Diospyros chinensis | - |
| Diospyros kaki | G5DAC7 | cv. Fupingjianshi, i.e. Diospyros chinensis | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains a potential N-linked glycosylation (N-X-S/T) site | Diospyros kaki |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis | Diospyros kaki |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | expression analysis of isozymes DkXTH1-5 during Fuping Jianshi fruit development, expression patterns, overview | Diospyros kaki | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH1 | Diospyros kaki | ? | - |
? | |
| additional information | no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH2 | Diospyros kaki | ? | - |
? | |
| tamarind xyloglucan | 3% tamarind xyloglucan | Diospyros kaki | ? | - |
? | |
| xyloglucan | - |
Diospyros kaki | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32550, sequence calculation | Diospyros kaki |
| ? | x * 34320, sequence calculation | Diospyros kaki |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DKXTH1 | - |
Diospyros kaki |
| DKXTH2 | - |
Diospyros kaki |
| XET | - |
Diospyros kaki |
| XTH | - |
Diospyros kaki |
| xyloglucan endotransglycosylase | - |
Diospyros kaki |
| xyloglucan endotransglycosylase/hydrolase | - |
Diospyros kaki |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Diospyros kaki |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | - |
Diospyros kaki |
| 5 | 5.5 | - |
Diospyros kaki |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | isozyme DkXTH1 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs when the pH ranges from pH 4 to pH 5, which is typical for XET isoenzymes | Diospyros kaki |
| 4 | 7 | isozyme DkXTH2 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs below pH 4, which is typical for XET isoenzymes | Diospyros kaki |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Diospyros kaki | sequence calculation | - |
8.66 |
| Diospyros kaki | sequence calculation | - |
9.28 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH | Diospyros kaki |
| additional information | three-dimensional structure modelling of DkXTH1 based on the template of the crystal structure of PttXET16A (PDB ID 1un1) | Diospyros kaki |
| additional information | three-dimensional structure modelling of DkXTH2 based on the template of the crystal structure of PttXET16A (PDB ID 1un1) | Diospyros kaki |
| physiological function | several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity | Diospyros kaki |
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