Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epicatechin gallate | - |
Petroselinum sp. | |
(-)-epigallocatechin gallate | non-specific | Petroselinum sp. | |
1-amino-4-hydroxy-2-methoxyanthracene-9,10-dione | - |
Petroselinum sp. | |
3-([3-(4-hydroxyphenyl)-3-oxopropanoyl]amino)-4-[methyl(octadecyl)amino]-benzoic acid | - |
Petroselinum sp. | |
4-([(4-methylphenyl)thio]methyl)-N-(2-pyridinylmethyl)benzamide | - |
Petroselinum sp. | |
4-chloromercuribenzoate | - |
Petroselinum sp. | |
6-bromo-2-hydroxy-1Hbenzo[de]isoquinoline-1,3-(2H)-dione | - |
Petroselinum sp. | |
aniline blue | - |
Petroselinum sp. | |
baicalein | - |
Petroselinum sp. | |
brilliant blue G | specific inhibition | Petroselinum sp. | |
Bromocresol green | - |
Petroselinum sp. | |
bromocresol purple | - |
Petroselinum sp. | |
ebselen | - |
Petroselinum sp. | |
eosin | - |
Petroselinum sp. | |
erythrosin | - |
Petroselinum sp. | |
hesperetin | - |
Petroselinum sp. | |
luteolin | - |
Petroselinum sp. | |
m-digallic acid | - |
Petroselinum sp. | |
additional information | high-throughput fluorescent dot-blot screening to search for enzyme inhibitors in vitro, detailed overview. Of 4216 xenobiotics tested, with cellulose-bound xyloglucan as donor-substrate, 18 inhibit XET activity and 18 promote it (especially anthraquinones and flavonoids). No compounds promotes XET in quantitative assays with (cellulose-free) soluble xyloglucan as substrate, suggesting that promotion is dependent on enzyme-cellulose interactions. With cellulose-free xyloglucan as substrate, 22 XET-inhibitors are identified, especially compounds that generate singlet oxygen. Inhibitors include tannins, sulphydryl reagent,s and triphenylmethanes | Petroselinum sp. | |
Mycophenolic acid | - |
Petroselinum sp. | |
N'-(1-E)-(5-bromofuran-2-yl)-methylidene-2-(3-methylphenoxy)-acetohydrazide | - |
Petroselinum sp. | |
Phenolphthalein | - |
Petroselinum sp. | |
phenylmercuric acetate | - |
Petroselinum sp. | |
phloretin | - |
Petroselinum sp. | |
retinoic acid | - |
Petroselinum sp. | |
riboflavin | the riboflavin inhibitory effect is light-dependent, non-specific | Petroselinum sp. | |
Tannic acid | - |
Petroselinum sp. | |
thiomersal | - |
Petroselinum sp. | |
vulpinic acid | specific inhibition | Petroselinum sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Petroselinum sp. | 5618 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | usage of cellulose-bound tamarind seed xyloglucan as donor substrate and [1-3H]XXXGol as acceptor substrate | Petroselinum sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XET | - |
Petroselinum sp. |
xyloglucan endotransglucosylase | - |
Petroselinum sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Petroselinum sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Petroselinum sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.027 | - |
pH 5.5, 20°C | Petroselinum sp. | eosin | |
0.029 | - |
pH 5.5, 20°C | Petroselinum sp. | riboflavin | |
0.036 | - |
pH 5.5, 20°C | Petroselinum sp. | erythrosin | |
0.082 | - |
pH 5.5, 20°C | Petroselinum sp. | Tannic acid | |
0.15 | - |
pH 5.5, 20°C | Petroselinum sp. | aniline blue | |
0.27 | - |
pH 5.5, 20°C | Petroselinum sp. | Bromocresol green | |
0.31 | - |
pH 5.5, 20°C | Petroselinum sp. | 4-chloromercuribenzoate | |
0.38 | - |
pH 5.5, 20°C | Petroselinum sp. | (-)-epigallocatechin gallate | |
0.43 | - |
pH 5.5, 20°C | Petroselinum sp. | (-)-epicatechin gallate | |
0.47 | - |
pH 5.5, 20°C | Petroselinum sp. | m-digallic acid | |
0.52 | - |
pH 5.5, 20°C | Petroselinum sp. | baicalein | |
0.52 | - |
pH 5.5, 20°C | Petroselinum sp. | Phenolphthalein | |
0.58 | - |
pH 5.5, 20°C | Petroselinum sp. | phloretin | |
0.61 | - |
pH 5.5, 20°C | Petroselinum sp. | hesperetin | |
0.62 | - |
pH 5.5, 20°C | Petroselinum sp. | vulpinic acid | |
0.7 | - |
pH 5.5, 20°C | Petroselinum sp. | luteolin |
General Information | Comment | Organism |
---|---|---|
evolution | xyloglucan endotransglucosylase (XET) is a homotransglycanase enzyme activity found in all land plants and in some charophytic algae | Petroselinum sp. |