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Literature summary for 2.4.1.207 extracted from
- Barley xyloglucan xyloglucosyl transferases bind xyloglucan-derived oligosaccharides in their acceptor-binding regions in multiple conformational states (2010), Arch. Biochem. Biophys., 496, 61-68.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare |  |
| 0.0053 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.0086 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.0103 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.0106 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.0113 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.0242 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.039 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.04 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | B1P1S7 | - |
- |
| Hordeum vulgare | P93671 | - |
- |
| Hordeum vulgare | P93672 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal affinity chromatography | Hordeum vulgare |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, at least 6 months, without loss of activity | Hordeum vulgare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + xyloglucan | - |
Hordeum vulgare | ? | - |
? | |
| donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | - |
Hordeum vulgare | ? | - |
? | |
| donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | - |
Hordeum vulgare | ? | - |
? | |
| donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | - |
Hordeum vulgare | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XET3 | isozyme | Hordeum vulgare |
| XET4 | isozyme | Hordeum vulgare |
| XET6 | isozyme | Hordeum vulgare |
| XTH | - |
Hordeum vulgare |
| xyloglucan endotransglycosylase | - |
Hordeum vulgare |
| xyloglucan endotransglycosylase/hydrolase | - |
Hordeum vulgare |
| xyloglucan xyloglucosyl transferases | - |
Hordeum vulgare |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.005 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.006 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.007 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.017 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.021 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.058 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.12 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.17 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.4 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.5 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET3, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 0.5 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 1 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 1 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET4, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 5 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 13 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
| 30 | - |
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp | isoform XET6, using xyloglycan as donor substrate, at 30°C, in 50 mM ammonium acetate buffer pH 6.0 | Hordeum vulgare | |
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