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Literature summary for 2.4.1.207 extracted from

  • Piens, K.; Henriksson, A.M.; Gullfot, F.; Lopez, M.; Faure, R.; Ibatullin, F.M.; Teeri, T.T.; Driguez, H.; Brumer, H.
    Glycosynthase activity of hybrid aspen xyloglucan endo-transglycosylase PttXET16-34 nucleophile mutants (2007), Org. Biomol. Chem., 5, 3971-3978.
    View publication on PubMed
Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Pichia pastoris strain GS115 Populus tremula x Populus tremuloides

Protein Variants

Protein Variants Comment Organism
E85A site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity Populus tremula x Populus tremuloides
E85G site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity Populus tremula x Populus tremuloides
E85S site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity Populus tremula x Populus tremuloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33780
-
 mutant PttXET16-34 E85G, calculated molecular mass Populus tremula x Populus tremuloides
33780
-
 mutant PttXET16-34 E85G, determined by ESI-TOF MS analysis Populus tremula x Populus tremuloides
33790
-
 mutant PttXET16-34 E85A, calculated molecular mass Populus tremula x Populus tremuloides
33790
-
 mutant PttXET16-34 E85A, determined by ESI-TOF MS analysis Populus tremula x Populus tremuloides
33810
-
 mutant PttXET16-34 E85S, calculated molecular mass Populus tremula x Populus tremuloides
33810
-
 mutant PttXET16-34 E85S, determined by ESI-TOF MS analysis Populus tremula x Populus tremuloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Populus tremula x Populus tremuloides breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan ?
-
 ?

Organism

Organism UniProt Comment Textmining
Populus tremula x Populus tremuloides Q8GZD5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Populus tremula x Populus tremuloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan Populus tremula x Populus tremuloides ?
-
 ?

Synonyms

Synonyms Comment Organism
PttXET
-
 Populus tremula x Populus tremuloides
PttXET16-34
-
 Populus tremula x Populus tremuloides
PttXET16A
-
 Populus tremula x Populus tremuloides
xyloglucan endo-transglycosylase
-
 Populus tremula x Populus tremuloides