Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | slightly activates | Pisum sativum | |
ascorbate | 10 mM, slightly activates | Pisum sativum | |
spermidine | 1 mM, slighly activates | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM; partial inhibition | Pisum sativum | |
Hg2+ | 1 mM, partial inhibition | Pisum sativum | |
La3+ | 10 mM, partial inhibition | Pisum sativum | |
additional information | not inhibited by 10 mM D-gluconolactone, chelating agents, cello-oligosaccharides, cellopentaose | Pisum sativum | |
Zn2+ | 10 mM, partial inhibition | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | xyloglucan-derived nonasaccharide | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | not ionically bound to the cell wall, little XET activity is covalently bound in the cell wall | Pisum sativum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, slightly activates | Pisum sativum | |
Mg2+ | 10 mM, slightly activates | Pisum sativum | |
Mn2+ | 10 mM, slightly activates | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + xyloglucan acceptor | Zea mays | - |
? | - |
? | |
donor xyloglucan + xyloglucan acceptor | Solanum lycopersicum | - |
? | - |
? | |
donor xyloglucan + xyloglucan acceptor | Pisum sativum | enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acer pseudoplatanus | - |
- |
- |
Allium schoenoprasum | - |
- |
- |
Anthriscus sylvestris | - |
- |
- |
Bromus erectus | - |
- |
- |
Holcus lanatus | - |
- |
- |
Lupinus polyphyllus | - |
- |
- |
Marchantia polymorpha | - |
- |
- |
Mnium hornum | - |
- |
- |
Pisum sativum | - |
- |
- |
Solanum lycopersicum | - |
cv. Ailsa Craig | - |
Taraxacum officinale | - |
- |
- |
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epicotyl | - |
Pisum sativum | - |
gametophyte | - |
Mnium hornum | - |
leaf | - |
Zea mays | - |
leaf | - |
Solanum lycopersicum | - |
leaf | - |
Lupinus polyphyllus | - |
leaf | - |
Holcus lanatus | - |
leaf | - |
Bromus erectus | - |
leaf | - |
Anthriscus sylvestris | - |
seedling | - |
Solanum lycopersicum | - |
seedling | 7-8-day etiolated seedlings | Pisum sativum | - |
stem | - |
Zea mays | - |
stem | - |
Solanum lycopersicum | - |
stem | - |
Holcus lanatus | - |
stem | - |
Bromus erectus | - |
stem | etiolated stem, activity is positively correlated with growth rate in different zones of pea stem | Pisum sativum | - |
stem | first year stem | Acer pseudoplatanus | - |
stem | peduncle | Taraxacum officinale | - |
stem | peduncle | Allium schoenoprasum | - |
thallus | - |
Marchantia polymorpha | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + acceptor xyloglucan | - |
Zea mays | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Solanum lycopersicum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Acer pseudoplatanus | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Taraxacum officinale | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Lupinus polyphyllus | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Marchantia polymorpha | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Mnium hornum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Allium schoenoprasum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Holcus lanatus | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Bromus erectus | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | - |
Anthriscus sylvestris | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | acceptor specificity | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | highly specific for xyloglucan as the glycosyl donor | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | minimum acceptor structure is Xyl2Glc3 | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | xyloglucan from Rosa cultures | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | xyloglucan from Tropaeolum majus seed | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | donor specificity | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers | Pisum sativum | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | acceptors: xyloglucan-derived nonasaccharide Glc4Xyl3GalFuc and certain other xyloglucan oligosaccharides, non-reducing terminal Xyl-Glc group is essential | Pisum sativum | ? | - |
? | |
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc | acceptor: xyloglucan-derived nonasaccharide XG9, Glc4Xyl3GalFuc, highly specific for xyloglucan as the glycosyl donor, xyloglucan from Tropaeolum seed is somewhat better than from Rosa cultures, enzyme transfers part of a large xyloglucan molecule to the nonasaccharide forming a polymer with a beta-1,4-linkage to the product and the acceptor group of the nonasaccharide is O-4 of the Glc residue furthest from the reducing terminus | Pisum sativum | ? | - |
? | |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc | XG9n, better substrate than Glc4Xyl3GalFuc | Pisum sativum | ? | - |
? | |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | XG7, better substrate than Glc4Xyl3GalFuc | Pisum sativum | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | - |
Zea mays | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | - |
Solanum lycopersicum | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan | Pisum sativum | ? | - |
? | |
additional information | - |
Zea mays | ? | - |
? | |
additional information | - |
Solanum lycopersicum | ? | - |
? | |
additional information | no acceptors: alpha-D-Xylp(1-6)D-Glc and isoprimeverose | Pisum sativum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pisum sativum |
25 | - |
assay at | Zea mays |
25 | - |
assay at | Solanum lycopersicum |
25 | - |
assay at | Acer pseudoplatanus |
25 | - |
assay at | Taraxacum officinale |
25 | - |
assay at | Lupinus polyphyllus |
25 | - |
assay at | Marchantia polymorpha |
25 | - |
assay at | Mnium hornum |
25 | - |
assay at | Allium schoenoprasum |
25 | - |
assay at | Holcus lanatus |
25 | - |
assay at | Bromus erectus |
25 | - |
assay at | Anthriscus sylvestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
boiling inactivates | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4: 25-35% of maximal activity, pH 7: 30-40% of maximal activity | Pisum sativum |