Data extracted from this reference:
KM Value [mM]
0.36
UDP-D-galactose
Helix pomatia
Localization
membrane
endomembrane-associated, probably in Golgi complex
Helix pomatia
Metals/Ions
MgCl2
10 mM, 30% stimulation
Helix pomatia
Molecular Weight [Da]
70000
x * 70000, SDS-PAGE
Helix pomatia
600000
greater than 600000 Da, gel filtration
Helix pomatia
Natural Substrates/ Products (Substrates)
UDPgalactose + galactogen
Helix pomatia
biosynthesis of galactogen
UDP + 1,6-beta-D-galactosylgalactogen
?
Organism
Purification (Commentary)
Source Tissue
albumen gland
Helix pomatia
Specific Activity [micromol/min/mg]
Storage Stability
-80°C, crude homogenate, 6 months
Helix pomatia
0°C, 24 h, 50% loss of activity
Helix pomatia
4°C, purified enzyme, stable for 1 week
Helix pomatia
Substrates and Products (Substrate)
UDP-D-galactose + arabinogalactan
arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylarabinogalactan
?
UDP-D-galactose + galactan
galactan from bovine lung 64% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactan
?
UDP-D-galactose + galactogen
galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
?
UDPgalactose + galactogen
biosynthesis of galactogen
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
?
Subunits
?
x * 70000, SDS-PAGE
Helix pomatia
pH Optimum
KM Value [mM] (protein specific)
0.36
UDP-D-galactose
Helix pomatia
Localization (protein specific)
membrane
endomembrane-associated, probably in Golgi complex
Helix pomatia
Metals/Ions (protein specific)
MgCl2
10 mM, 30% stimulation
Helix pomatia
Molecular Weight [Da] (protein specific)
70000
x * 70000, SDS-PAGE
Helix pomatia
600000
greater than 600000 Da, gel filtration
Helix pomatia
Natural Substrates/ Products (Substrates) (protein specific)
UDPgalactose + galactogen
Helix pomatia
biosynthesis of galactogen
UDP + 1,6-beta-D-galactosylgalactogen
?
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
albumen gland
Helix pomatia
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
-80°C, crude homogenate, 6 months
Helix pomatia
0°C, 24 h, 50% loss of activity
Helix pomatia
4°C, purified enzyme, stable for 1 week
Helix pomatia
Substrates and Products (Substrate) (protein specific)
UDP-D-galactose + arabinogalactan
arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylarabinogalactan
?
UDP-D-galactose + galactan
galactan from bovine lung 64% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactan
?
UDP-D-galactose + galactogen
galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
?
UDPgalactose + galactogen
biosynthesis of galactogen
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
?
Subunits (protein specific)
?
x * 70000, SDS-PAGE
Helix pomatia
pH Optimum (protein specific)
Other publictions for EC 2.4.1.205
488883
Goudsmit
Biosynthesis of galactogen: id ...
Helix pomatia
Biochim. Biophys. Acta
992
289-297
1989
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