BRENDA - Enzyme Database
show all sequences of 2.4.1.205

Biosynthesis of galactogen: identification of a beta-(1->6)-D-galactosyltransferase in Helix pomatia albumen glands

Goudsmit, E.M.; Ketchum, P.A.; Grossens, M.K.; Blake, D.A.; Biochim. Biophys. Acta 992, 289-297 (1989) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
UDP-D-galactose
-
Helix pomatia
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
endomembrane-associated, probably in Golgi complex
Helix pomatia
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
MgCl2
10 mM, 30% stimulation
Helix pomatia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
x * 70000, SDS-PAGE
Helix pomatia
600000
-
greater than 600000 Da, gel filtration
Helix pomatia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPgalactose + galactogen
Helix pomatia
biosynthesis of galactogen
UDP + 1,6-beta-D-galactosylgalactogen
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Helix pomatia
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Helix pomatia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
albumen gland
-
Helix pomatia
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.466
-
-
Helix pomatia
Storage Stability
Storage Stability
Organism
-80°C, crude homogenate, 6 months
Helix pomatia
0°C, 24 h, 50% loss of activity
Helix pomatia
4°C, purified enzyme, stable for 1 week
Helix pomatia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-D-galactose + arabinogalactan
arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylarabinogalactan
-
-
-
?
UDP-D-galactose + galactan
galactan from bovine lung 64% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactan
-
-
-
?
UDP-D-galactose + galactogen
galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
-
-
-
?
UDPgalactose + galactogen
biosynthesis of galactogen
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 70000, SDS-PAGE
Helix pomatia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.5
-
Helix pomatia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
UDP-D-galactose
-
Helix pomatia
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
endomembrane-associated, probably in Golgi complex
Helix pomatia
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
MgCl2
10 mM, 30% stimulation
Helix pomatia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
x * 70000, SDS-PAGE
Helix pomatia
600000
-
greater than 600000 Da, gel filtration
Helix pomatia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPgalactose + galactogen
Helix pomatia
biosynthesis of galactogen
UDP + 1,6-beta-D-galactosylgalactogen
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Helix pomatia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
albumen gland
-
Helix pomatia
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.466
-
-
Helix pomatia
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, crude homogenate, 6 months
Helix pomatia
0°C, 24 h, 50% loss of activity
Helix pomatia
4°C, purified enzyme, stable for 1 week
Helix pomatia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-D-galactose + arabinogalactan
arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylarabinogalactan
-
-
-
?
UDP-D-galactose + galactan
galactan from bovine lung 64% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactan
-
-
-
?
UDP-D-galactose + galactogen
galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
-
-
-
?
UDPgalactose + galactogen
biosynthesis of galactogen
488883
Helix pomatia
UDP + 1,6-beta-D-galactosylgalactogen
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 70000, SDS-PAGE
Helix pomatia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
7.5
-
Helix pomatia
Other publictions for EC 2.4.1.205
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
488883
Goudsmit
Biosynthesis of galactogen: id ...
Helix pomatia
Biochim. Biophys. Acta
992
289-297
1989
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1
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1
1
1
2
1
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1
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1
1
3
4
1
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1
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