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Literature summary for 2.4.1.203 extracted from

  • Smehilova, M.; Dobruskova, J.; Novak, O.; Takac, T.; Galuszka, P.
    Cytokinin-specific glycosyltransferases possess different roles in cytokinin homeostasis maintenance (2016), Front. Plant Sci., 7, 1264 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene UGT76C1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana Arabidopsis thaliana
gene UGT76C2, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana Arabidopsis thaliana
gene UGT85A1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of the T-DNA insertion ugt76c1-1 loss-of-function mutant Arabidopsis thaliana
additional information generation of the T-DNA insertion ugt76c2-1 loss-of-function mutant Arabidopsis thaliana
additional information generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol in silico localization analysis Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + trans-zeatin Arabidopsis thaliana
-
UDP + 7-beta-D-glucosyl-trans-zeatin
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana additional information analysis of CK glucosides formation during main developmental transition phases
-
Arabidopsis thaliana Q9FI99
-
-
Arabidopsis thaliana Q9FIA0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf UGT85A1 is specifically expressed in senescent leaves Arabidopsis thaliana
-
additional information analysis of CK glucosides formation during main developmental transition phases Arabidopsis thaliana
-
additional information UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme UGT85A1 is also active with cis-zeatin Arabidopsis thaliana ?
-
-
additional information enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215 Arabidopsis thaliana ?
-
-
UDP-glucose + trans-zeatin
-
Arabidopsis thaliana UDP + 7-beta-D-glucosyl-trans-zeatin
-
?

Synonyms

Synonyms Comment Organism
CK N-specific UGT76C1 glycosyltransferase
-
Arabidopsis thaliana
CK-specific UGT
-
Arabidopsis thaliana
cytokinin-N-glucosyltransferase 1 UniProt Arabidopsis thaliana
cytokinin-N-glucosyltransferase 2 UniProt Arabidopsis thaliana
cytokinin-specific glycosyltransferase
-
Arabidopsis thaliana
More see also EC 2.4.1.215 Arabidopsis thaliana
UDP-glycosyltransferase 76C1 UniProt Arabidopsis thaliana
UDP-glycosyltransferase 76C2 UniProt Arabidopsis thaliana
ugt76c1
-
Arabidopsis thaliana
ugt76c2
-
Arabidopsis thaliana
UGT85A1
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the CK N-specific UGT76C1 glycosyltransferase shows no response to any of the cytokinins on transcript level additional information
Arabidopsis thaliana enzyme UGT85A1 is sensitive only to trans-zeatin treatment which increases its expression mildly by 1.59times up
Arabidopsis thaliana UGT76C2 is activated rapidly in response to exogenously applied cytokinin, extremely elevated trans-zeatin (tZ)-type CKs is observed up

General Information

General Information Comment Organism
malfunction a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview Arabidopsis thaliana
malfunction a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview Arabidopsis thaliana
malfunction UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview Arabidopsis thaliana
physiological function cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol Arabidopsis thaliana