Cloned (Comment) | Organism |
---|---|
gene UGT76C1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana | Arabidopsis thaliana |
gene UGT76C2, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana | Arabidopsis thaliana |
gene UGT85A1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the T-DNA insertion ugt76c1-1 loss-of-function mutant | Arabidopsis thaliana |
additional information | generation of the T-DNA insertion ugt76c2-1 loss-of-function mutant | Arabidopsis thaliana |
additional information | generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in silico localization analysis | Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + trans-zeatin | Arabidopsis thaliana | - |
UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | additional information | analysis of CK glucosides formation during main developmental transition phases | - |
Arabidopsis thaliana | Q9FI99 | - |
- |
Arabidopsis thaliana | Q9FIA0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | UGT85A1 is specifically expressed in senescent leaves | Arabidopsis thaliana | - |
additional information | analysis of CK glucosides formation during main developmental transition phases | Arabidopsis thaliana | - |
additional information | UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme UGT85A1 is also active with cis-zeatin | Arabidopsis thaliana | ? | - |
- |
|
additional information | enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215 | Arabidopsis thaliana | ? | - |
- |
|
UDP-glucose + trans-zeatin | - |
Arabidopsis thaliana | UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CK N-specific UGT76C1 glycosyltransferase | - |
Arabidopsis thaliana |
CK-specific UGT | - |
Arabidopsis thaliana |
cytokinin-N-glucosyltransferase 1 | UniProt | Arabidopsis thaliana |
cytokinin-N-glucosyltransferase 2 | UniProt | Arabidopsis thaliana |
cytokinin-specific glycosyltransferase | - |
Arabidopsis thaliana |
More | see also EC 2.4.1.215 | Arabidopsis thaliana |
UDP-glycosyltransferase 76C1 | UniProt | Arabidopsis thaliana |
UDP-glycosyltransferase 76C2 | UniProt | Arabidopsis thaliana |
ugt76c1 | - |
Arabidopsis thaliana |
ugt76c2 | - |
Arabidopsis thaliana |
UGT85A1 | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | the CK N-specific UGT76C1 glycosyltransferase shows no response to any of the cytokinins on transcript level | additional information |
Arabidopsis thaliana | enzyme UGT85A1 is sensitive only to trans-zeatin treatment which increases its expression mildly by 1.59times | up |
Arabidopsis thaliana | UGT76C2 is activated rapidly in response to exogenously applied cytokinin, extremely elevated trans-zeatin (tZ)-type CKs is observed | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview | Arabidopsis thaliana |
malfunction | a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview | Arabidopsis thaliana |
malfunction | UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview | Arabidopsis thaliana |
physiological function | cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol | Arabidopsis thaliana |