Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + trans-zeatin | Brassica napus | - |
UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | Leptosphaeria maculans | - |
UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | Brassica napus | - |
UDP + 9-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | Leptosphaeria maculans v23.1.3 | - |
UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | Leptosphaeria maculans JN3 | - |
UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Leptosphaeria maculans | - |
- |
- |
Leptosphaeria maculans JN3 | - |
- |
- |
Leptosphaeria maculans v23.1.3 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica napus | - |
mycelium | - |
Leptosphaeria maculans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + trans-zeatin | - |
Brassica napus | UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | - |
Leptosphaeria maculans | UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | - |
Leptosphaeria maculans v23.1.3 | UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | - |
Leptosphaeria maculans JN3 | UDP + 7-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | - |
Brassica napus | UDP + 9-beta-D-glucosyl-trans-zeatin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trans-zeatin O-glucosyltransferase | - |
Leptosphaeria maculans |
tZOG | - |
Leptosphaeria maculans |
Organism | Comment | Expression |
---|---|---|
Brassica napus | Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves | up |
General Information | Comment | Organism |
---|---|---|
metabolism | in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview | Brassica napus |
metabolism | the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans | Leptosphaeria maculans |