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Literature summary for 2.4.1.203 extracted from

  • Zhou, Y.; Li, W.; You, W.; Di, Z.; Wang, M.; Zhou, H.; Yuan, S.; Wong, N.K.; Xiao, Y.
    Discovery of Arabidopsis UGT73C1 as a steviol-catalyzing UDP-glycosyltransferase with chemical probes (2018), Chem. Commun. (Camb.), 54, 7179-7182 .
    View publication on PubMed
Search for more literature for 2.4.1.203

Cloned(Commentary)

Cloned (Comment) Organism
gene UGT73C1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9ZQ99
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol Arabidopsis thaliana ?
-
-
UDP-alpha-D-glucose + 2-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]ethyl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
-
 Arabidopsis thaliana UDP + ?
-
 ?
UDP-alpha-D-glucose + prop-2-yn-1-yl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate determination and analysis of the substrate binding structure of UGT73C1, model, overview Arabidopsis thaliana UDP + ?
-
 ?
UDP-glucose + steviol determination and analysis of the substrate binding structure of UGT73C1, model, overview Arabidopsis thaliana UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
AtZOG1 UniProt Arabidopsis thaliana
cytokinin-O-glucosyltransferase 1 UniProt Arabidopsis thaliana
UDP-glycosyltransferase 73C1 UniProt Arabidopsis thaliana
UGT73C1
-
 Arabidopsis thaliana
zeatin O-glucosyltransferase 1 UniProt Arabidopsis thaliana
ZOG1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
additional information homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates Arabidopsis thaliana