Cloned (Comment) | Organism |
---|---|
gene UGT85A1, overexpression of the wild-type enzyme in Arabidopsis thaliana using transfection via Agrobacterium tumefaciens strain GV3101, expression as GFP-tagged enzyme in onion epidermis by particle bombardment | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + trans-zeatin | Arabidopsis thaliana | - |
UDP + O-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | Arabidopsis thaliana Col-0 | - |
UDP + O-beta-D-glucosyl-trans-zeatin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SK82 | - |
- |
Arabidopsis thaliana Col-0 | Q9SK82 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | UGT85A1 is mainly expressed in the early seedlings, spatial-temporal expression patterns, overview | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + trans-zeatin | - |
Arabidopsis thaliana | UDP + O-beta-D-glucosyl-trans-zeatin | - |
? | |
UDP-glucose + trans-zeatin | - |
Arabidopsis thaliana Col-0 | UDP + O-beta-D-glucosyl-trans-zeatin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucosyltransferase UGT85A1 | - |
Arabidopsis thaliana |
trans-zeatin O-glucosyltransferase | - |
Arabidopsis thaliana |
UGT85A1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview | Arabidopsis thaliana |
physiological function | glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview | Arabidopsis thaliana |