Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, stimulation of 79% | Zea mays | |
ascorbate | at 5 mM, stimulation of 42% | Zea mays | |
dithioerythritol | 5 mM, stimulation of 96% | Zea mays | |
glutathione | 5 mM, stimulation of 92% | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 3% remaining activity | Zea mays | |
EDTA | 16% decrease of activity | Zea mays | |
Fe2+ | 5 mM, 5% remaining activity | Zea mays | |
N-ethylmaleimide | at 1 mM, 96% decrease of activity, after addition of dithioerythritol, full activity | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.174 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | enzyme in peak 2 | Zea mays | |
0.217 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | enzyme in peak 1 | Zea mays | |
0.28 | - |
UDPglucose | reaction with 2,4-dihydroxy-1,4-benzoxazin-3-one, enzyme in peak 2 | Zea mays | |
0.286 | - |
UDPglucose | reaction with 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, enzyme in peak 1 | Zea mays | |
0.638 | - |
2,4-dihydroxy-1,4-benzoxazin-3-one | enzyme in peak 2 | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, stimulation | Zea mays | |
MgCl2 | 5 mM, stimulation | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration, both peaks | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | Zea mays | - |
UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
two different enzymes with different substrate specificities, genes Bx8 and Bx9 | - |
Purification (Comment) | Organism |
---|---|
partial, activity elutes from Q-Sepharose column in two peaks, named peak 1 and peak 2, exhibiting different activities towards substrates | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.218 | - |
enzyme in peak 1 and peak 2 | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2,4-dihydroxy-1,4-benzoxazin-3-one | peak 1 glucosyltransferase has 3.6% of activity compared to activity towards 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, peak 2 glucosyltransferase has 57% of activity compared to activity towards 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | Zea mays | UDP + 2,4-dihydroxy-2H-1,4-benzoxazin-3-one 2-D-glucoside | - |
? | |
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | - |
Zea mays | UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zea mays |
45 | - |
- |
Zea mays |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 88% of maximal activity at 55°C, 60% of maximal activity at 25°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | 10% of maximal activity at pH 6.5 and 30% of maximal activity at pH 10.0 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | at 5 mM, stimulation of 42% | Zea mays |