Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Zea mays |
transfected into Arabidopsis | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one | BX8-gene encoded protein | Zea mays | |
0.071 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | BX9-gene encoded protein | Zea mays | |
0.081 | - |
UDP-glucose | BX8-gene encoded protein | Zea mays | |
0.081 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | BX8-gene encoded protein | Zea mays | |
0.096 | - |
UDP-glucose | BX9-gene encoded protein | Zea mays | |
1.3 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one | BX9-gene encoded protein | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | Zea mays | involved in detoxification of benzoxazinoids | UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
two different enzymes with different substrate specificities, genes Bx8 and Bx9 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein | Zea mays |
0.5 | - |
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein | Zea mays |
1.4 | - |
2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX8-gene encoded protein | Zea mays |
2.6 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein | Zea mays |
6 | - |
2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, BX9-gene encoded protein | Zea mays |
13 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX9-gene encoded protein | Zea mays |
13.4 | - |
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein | Zea mays |
24 | - |
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one as substrate, BX8-gene encoded protein | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one | - |
Zea mays | UDP + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? | |
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | - |
Zea mays | UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? | |
UDP-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | involved in detoxification of benzoxazinoids | Zea mays | UDP + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? | |
UDP-glucose + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one | low glucosylation efficiency | Zea mays | UDP + 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? | |
UDP-glucose + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one | - |
Zea mays | UDP + 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucoside | - |
? |