Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermosipho africanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
cellopentaose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.062 | - |
cellotetraose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.094 | - |
cellotriose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.12 | - |
cellobiose | at pH 7.5 and 75°C | Thermosipho africanus | |
1.57 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 75°C | Thermosipho africanus | |
14.3 | - |
D-glucose | at pH 7.5 and 75°C | Thermosipho africanus | |
25.7 | - |
D-xylose | at pH 7.5 and 75°C | Thermosipho africanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Thermosipho africanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + D-glucose | Thermosipho africanus | - |
cellobiose + phosphate | - |
r | |
cellobiose + phosphate | Thermosipho africanus | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosipho africanus | B7IED6 | - |
- |
Thermosipho africanus TCF52B | B7IED6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating column chromatography and HiTrap Q column chromatography | Thermosipho africanus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
with D-maltose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.22 | - |
with D-arabinose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.22 | - |
with D-fructose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.22 | - |
with N-acetyl-D-glucosamine as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.27 | - |
with D-galactose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.35 | - |
with D-xylose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.38 | - |
with D-mannose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
0.85 | - |
with 2-deoxy-D-glucose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
1.08 | - |
with D-glucosamine as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
1.86 | - |
with D-glucose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
49.98 | - |
with gentiobiose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
58.61 | - |
with cellobiose as substrate, at pH 7.5 and 75°C | Thermosipho africanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-arabinose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-fructose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-galactose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-glucosamine | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-glucose | - |
Thermosipho africanus | cellobiose + phosphate | - |
r | |
alpha-D-glucose 1-phosphate + D-maltose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-mannose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + D-xylose | - |
Thermosipho africanus | phosphate + ? | - |
r | |
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine | - |
Thermosipho africanus | phosphate + ? | - |
r | |
cellobiose + phosphate | - |
Thermosipho africanus | alpha-D-glucose 1-phosphate + D-glucose | - |
r | |
cellobiose + phosphate | 38.6% activity compared to cellotetraose | Thermosipho africanus | alpha-D-glucose 1-phosphate + D-glucose | - |
r | |
cellopentaose + phosphate | 63.3% activity compared to cellotetraose | Thermosipho africanus | ? | - |
r | |
cellotetraose + phosphate | 100% activity | Thermosipho africanus | ? | - |
r | |
cellotriose + phosphate | 89.7% activity compared to cellotetraose | Thermosipho africanus | ? | - |
r | |
gentiobiose + phosphate | - |
Thermosipho africanus | ? | - |
r | |
additional information | beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate | Thermosipho africanus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Thermosipho africanus |
Synonyms | Comment | Organism |
---|---|---|
Cbp | - |
Thermosipho africanus |
THA_1941 | - |
Thermosipho africanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermosipho africanus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | the enzyme shows more than 50% activity between 50 and 90°C | Thermosipho africanus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the enzyme retains 80% of its initial activity after incubating at 75°C and pH 7.5 for 30 min | Thermosipho africanus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
cellobiose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.28 | - |
cellotriose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.34 | - |
cellopentaose | at pH 7.5 and 75°C | Thermosipho africanus | |
0.49 | - |
cellotetraose | at pH 7.5 and 75°C | Thermosipho africanus | |
1.37 | - |
D-xylose | at pH 7.5 and 75°C | Thermosipho africanus | |
7.61 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 75°C | Thermosipho africanus | |
30.3 | - |
D-glucose | at pH 7.5 and 75°C | Thermosipho africanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Thermosipho africanus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9.5 | the enzyme shows more than 50% activity between pH 4.0 and 9.5 | Thermosipho africanus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-xylose | at pH 7.5 and 75°C | Thermosipho africanus | |
1.92 | - |
cellobiose | at pH 7.5 and 75°C | Thermosipho africanus | |
2.12 | - |
D-glucose | at pH 7.5 and 75°C | Thermosipho africanus | |
2.98 | - |
cellotriose | at pH 7.5 and 75°C | Thermosipho africanus | |
4.85 | - |
alpha-D-glucose 1-phosphate | at pH 7.5 and 75°C | Thermosipho africanus | |
5.67 | - |
cellopentaose | at pH 7.5 and 75°C | Thermosipho africanus | |
7.91 | - |
cellotetraose | at pH 7.5 and 75°C | Thermosipho africanus |