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Literature summary for 2.4.1.20 extracted from

  • Kitaoka, M.; Nomura, S.; Yoshida, M.; Hayashi, K.
    Reaction on D-glucal by an inverting phosphorylase to synthesize derivatives of 2-deoxy-beta-D-arabino-hexopyranosyl-(1-->4)-D-glucose (2II-deoxycellobiose) (2006), Carbohydr. Res., 341, 545-549.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Cellulomonas gilvus

Organism

Organism UniProt Comment Textmining
Cellulomonas gilvus
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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the activity on D-glucal is approximately 500times less than that on alpha-D-glucose 1-phosphate at 10 mM of each donor with 10 mM D-glucose by comparing the initial rate of the production Cellulomonas gilvus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucal + 2-deoxy-2-(hydroxymethyl)-beta-D-xylopyranose
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Cellulomonas gilvus 2-deoxy-3-O-(2-deoxy-beta-D-arabino-hexopyranosyl)-2-(hydroxymethyl)-beta-D-xylopyranose + ?
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r
D-glucal + D-glucose
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Cellulomonas gilvus 2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-glucose + ?
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r
D-glucal + D-mannose
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Cellulomonas gilvus 2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-mannose + ?
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r
D-glucal + D-xylose
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Cellulomonas gilvus 2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-xylose + ?
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r

Synonyms

Synonyms Comment Organism
Cbp cellobiose phosphorylase Cellulomonas gilvus