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Literature summary for 2.4.1.20 extracted from
- Role of non-covalent enzyme-substrate interactions in the reaction catalysed by cellobiose phosphorylase from Cellulomonas uda (2000), Biochem. J., 351, 649-659.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-deoxy-D-glucose | competitive with D-glucose, dead end inhibitor | Cellulomonas uda |  |
| 4-thio-cellobiose | weak inhibition in both directions of reaction | Cellulomonas uda | |
| cellobiose | substrate inhibition, competitive with glucose-1-phosphate | Cellulomonas uda | |
| D-glucose | mixed type inhibition against cellobiose, pure beta-D-glucose inhibits stronger than mixture of both anomers | Cellulomonas uda | |
| D-glucose 1-phosphate | substrate inhibition, competitive with cellobiose | Cellulomonas uda | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
phosphate | - |
Cellulomonas uda | |
| 2.15 | - |
alpha-D-glucose 1-phosphate | - |
Cellulomonas uda | |
| 2.26 | - |
D-glucose | - |
Cellulomonas uda | |
| 4.3 | - |
cellobiose | - |
Cellulomonas uda | |
| 7.4 | - |
6-deoxy-6-fluoro-D-glucose | - |
Cellulomonas uda | |
| 10.2 | - |
6-deoxy-D-glucose | - |
Cellulomonas uda | |
| 10.8 | - |
D-glucoseamine | - |
Cellulomonas uda | |
| 15.9 | - |
D-xylose | - |
Cellulomonas uda | |
| 17.2 | - |
2-deoxy-2-fluoro-D-glucose | - |
Cellulomonas uda | |
| 24.3 | - |
2-deoxy-D-glucose | - |
Cellulomonas uda | |
| 27.3 | - |
D-mannose | - |
Cellulomonas uda | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + phosphate | Cellulomonas uda | important for energy efficient catabolism of cellobiose in cytoplasm | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas uda | - |
- |
- |
| Cellulomonas uda | - |
DSM 20108 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D -glucose 1-fluoride + D-glucose | - |
Cellulomonas uda | cellobiose + fluoride | - |
ir | |
| alpha-D-glucose 1-phosphate + 2-deoxy-2-fluoro-D-glucose | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 6-deoxy-6-fluoro-D-glucose | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-glucosamine | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-glucose | - |
Cellulomonas uda | cellobiose + phosphate | - |
? | |
| alpha-D-glucose 1-phosphate + D-mannose | - |
Cellulomonas uda | ? | - |
? | |
| alpha-D-glucose 1-phosphate + D-xylose | - |
Cellulomonas uda | ? | - |
? | |
| beta-D-glucose 1-phosphate + alpha-D-glucopyranosyl fluoride | beta-D-glucopyranosyl fluoride is not ulilized | Cellulomonas uda | beta-cellobiose + HF | - |
? | |
| cellobiose + arsenate | - |
Cellulomonas uda | D-glucose + alpha-D-glucose 1-arsenate | alpha-D-glucose 1-arsenate hydrolyses spontaneously to form D-glucose and arsenate | ir | |
| cellobiose + phosphate | beta-cellobiose preferred over alpha-anomer | Cellulomonas uda | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | ternary-complex kinetic mechanism | Cellulomonas uda | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| cellobiose + phosphate | important for energy efficient catabolism of cellobiose in cytoplasm | Cellulomonas uda | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CuCbP | - |
Cellulomonas uda |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
D-glucoseamine | - |
Cellulomonas uda | |
| 6.6 | - |
D-mannose | - |
Cellulomonas uda | |
| 8 | - |
2-deoxy-2-fluoro-D-glucose | - |
Cellulomonas uda | |
| 8.7 | - |
D-xylose | - |
Cellulomonas uda | |
| 11.6 | - |
2-deoxy-D-glucose | - |
Cellulomonas uda | |
| 29.1 | - |
cellobiose | - |
Cellulomonas uda | |
| 43.2 | - |
D-glucose | - |
Cellulomonas uda | |
| 44.6 | - |
6-deoxy-6-fluoro-D-glucose | - |
Cellulomonas uda | |
| 55.3 | - |
6-deoxy-D-glucose | - |
Cellulomonas uda | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
D-glucose | - |
Cellulomonas uda | |
| 3.2 | - |
D-glucose 1-phosphate | - |
Cellulomonas uda | |
| 4.4 | - |
4-deoxy-D-glucose | - |
Cellulomonas uda | |
| 13 | - |
4-thio-cellobiose | in presence of saturating concentrations of phosphate | Cellulomonas uda | |
| 14.8 | - |
cellobiose | - |
Cellulomonas uda | |
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