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Literature summary for 2.4.1.20 extracted from

  • Tanaka, K.; Kawaguchi, T.; Imada, Y.; Ooi, T.; Arai, M.
    Purification and properties of cellobiose phosphorylase from Clostridium thermocellum (1995), J. Ferment. Bioeng., 79, 212-216.
No PubMed abstract available
Search for more literature for 2.4.1.20

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose strong inhibition at high concentrations Acetivibrio thermocellus
N-ethylmaleimide
-
 Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
 alpha-D-glucose 1-phosphate
-
 Acetivibrio thermocellus
10
-
 D-glucosamine
-
 Acetivibrio thermocellus
40
-
 D-xylose
-
 Acetivibrio thermocellus
170
-
 D-mannose
-
 Acetivibrio thermocellus
250
-
 2-deoxy-D-glucose
-
 Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ not required Acetivibrio thermocellus
additional information no influence of metal ions on reaction Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
 SDS-PAGE Acetivibrio thermocellus
150000
-
 gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Acetivibrio thermocellus
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
 strain ATCC 27405
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 purified enzyme Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Acetivibrio thermocellus ?
-
 ?
alpha-D-glucose 1-phosphate + D-arabinose very poor substrate Acetivibrio thermocellus ?
-
 ?
alpha-D-glucose 1-phosphate + D-glucosamine
-
 Acetivibrio thermocellus ?
-
 ?
alpha-D-glucose 1-phosphate + D-mannose
-
 Acetivibrio thermocellus ?
-
 ?
alpha-D-glucose 1-phosphate + D-xylose
-
 Acetivibrio thermocellus ?
-
 ?
cellobiose + phosphate
-
 Acetivibrio thermocellus alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 85000, gel filtration, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 very low activity at 20 and 80°C Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 unstable above Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
 Acetivibrio thermocellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 most stable at pH 7, rapid loss of activity at different pH Acetivibrio thermocellus