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Literature summary for 2.4.1.20 extracted from

  • Kitaoka, M.; Sasaki, T.; Taniguchi, H.
    Phosphorolytic reaction of Cellvibrio gilvus cellobiose phosphorylase (1992), Biosci. Biotechnol. Biochem., 56, 652-655.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cellobiose
-
Cellulomonas gilvus
D-cellobiose
-
Cellulomonas gilvus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
phosphate
-
Cellulomonas gilvus
2.6
-
D-cellobiose
-
Cellulomonas gilvus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
SDS-PAGE Cellulomonas gilvus
180000
-
gel filtration Cellulomonas gilvus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Cellulomonas gilvus
-
alpha-D-glucose 1-phosphate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Cellulomonas gilvus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cellulomonas gilvus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.8
-
purified enzyme Cellulomonas gilvus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + phosphate
-
Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose
-
?
cellobiose + phosphate sequential bi-bi mechanism Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 85000, gel filtration, SDS-PAGE Cellulomonas gilvus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
forward and reverse reaction Cellulomonas gilvus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.8
-
D-cellobiose
-
Cellulomonas gilvus