Literature summary for 2.4.1.199 extracted from

Yokoyama, K.; Ballou, C.E.
Synthesis of alpha 1-6-mannooligosaccharides in Mycobacterium smegmatis. Function of beta-mannosylphosphoryldecaprenol as the mannosyl donor (1989), J. Biol. Chem., 264, 21621-21628.

Search for more literature for 2.4.1.199

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2	-	1,6-alpha-D-mannotriose	-	Mycolicibacterium smegmatis
2.4	-	1,6-alpha-D-mannotetraose	-	Mycolicibacterium smegmatis
2.6	-	1,6-alpha-D-mannopentaose	-	Mycolicibacterium smegmatis
28.6	-	methyl-alpha-D-mannoside	-	Mycolicibacterium smegmatis
35.7	-	1,6-alpha-D-mannobiose	-	Mycolicibacterium smegmatis
86.9	-	D-mannose	-	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Mycolicibacterium smegmatis	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
mannosylphosphodecaprenol + 1,6-alpha-D-mannooligosaccharides	Mycolicibacterium smegmatis	involved in the formation of mannooligosaccharides in membrane of Mycobacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosyl-1,6-alpha-D-mannooligosaccharides	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-mannosylphosphodecaprenol + 1,6-alpha-D-mannobiose	-	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosyltriose	-	?
beta-mannosylphosphodecaprenol + D-mannose	-	Mycolicibacterium smegmatis	?	-	?
mannosylphosphodecaprenol + 1,6-alpha-D-mannooligosaccharides	poor substrates are mannose and 1,6-alpha-mannobiose, no substrates are 1,2-, 1,3-alpha-mannooligosaccharides or myo-inositol-2,6-dimannoside	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosyl-1,6-alpha-D-mannooligosaccharides	-	?
mannosylphosphodecaprenol + 1,6-alpha-D-mannooligosaccharides	involved in the formation of mannooligosaccharides in membrane of Mycobacterium smegmatis	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosyl-1,6-alpha-D-mannooligosaccharides	-	?
mannosylphosphodecaprenol + 1,6-alpha-D-mannopentaose	-	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannohexaose	-	?
mannosylphosphodecaprenol + 1,6-alpha-D-mannotetraose	-	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosylpentaose	-	?
mannosylphosphodecaprenol + 1,6-alpha-D-mannotriose	-	Mycolicibacterium smegmatis	decaprenol phosphate + 1,6-alpha-D-mannosyltetraose	-	?
mannosylphosphodecaprenol + methyl-alpha-D-mannoside	-	Mycolicibacterium smegmatis	decaprenol phosphate + ?	-	?
mannosylphosphodecaprenol + myo-inositol-trimannoside	with an 1,6-alpha-mannobiose unit as part of its structure	Mycolicibacterium smegmatis	decaprenol phosphate + ?	-	?
additional information	substrate specificity	Mycolicibacterium smegmatis	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)