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Literature summary for 2.4.1.198 extracted from

  • David, R.; Talbot, E.; Allen, B.; Wilson, A.; Arshad, U.; Doherty, A.
    The development of an in vitro Pig-a assay in L5178Y cells (2018), Arch. Toxicol., 92, 1609-1623 .
    View publication on PubMed
Search for more literature for 2.4.1.198

Cloned(Commentary)

Cloned (Comment) Organism
gene Pig-a, DNA and amino acid sequence determination and analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information GPI anchor deficiency is most often caused by mutation in the Pig-a gene, thus measuring GPI anchor deficiency is considered to be almost equivalent to measuring Pig-a mutation. Development and validation of an in vitro Pig-a assay in L5178Y mouse lymphoma cells, method optimization, overview. Antibodies against GPI-linked CD90.2 and stably expressed CD45 are used to determine GPI-status by flow cytometry, antibody concentration and incubation times are optimized. The optimum phenotypic expression period is 8 days. Application of the in vitro Pig-a assay to a selection of well-validated genotoxic and non-genotoxic compounds. EMS, N-ethyl-N-nitrosourea, and 4-nitroquinoline-N-oxide dose dependently increase the numbers of GPI(-) cells, while etoposide, mitomycin C, and a bacterial-specific mutagen do not dependently increase the numbers of GPI(-) cells Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol Mus musculus
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 UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q64323
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-

Source Tissue

Source Tissue Comment Organism Textmining
L-5178-Y cell mouse lymphoma cells Mus musculus
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lymphoma cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol
-
 Mus musculus UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
-
 ?

Synonyms

Synonyms Comment Organism
PigA
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 Mus musculus

General Information

General Information Comment Organism
malfunction mutations in Pig-a prevent GPI-anchor synthesis resulting in loss of cell-surface GPI-linked proteins Mus musculus
metabolism the enzyme is involved in the glycophosphatidylinositol (GPI) anchor synthesis pathway which is highly conserved between species Mus musculus
physiological function hemizygous phosphatidylinositol class A (Pig-a) forms the catalytic subunit of N-acetylglucosaminyltransferase that is required for glycophosphatidylinositol (GPI) anchor biosynthesis Mus musculus