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Literature summary for 2.4.1.198 extracted from

  • Kostova, Z.; Rancour, D.M.; Menon, A.K.; Orlean, P.
    Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies Gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis (2000), Biochem. J., 350, 815-822.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of PIG-A/GPI3 and PIG-C/GPI2 as FLAG-tagged fusion proteins in yeast, expression of FLAG-tagged PIG-A/GPI3 in Escherichia coli Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Saccharomyces cerevisiae 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
enzyme exists as a complex of at least 3 proteins PIG-A or GPI3, PIG-C or GPI2 and GPI1, in which PIG-A, not PIG-C, is the substrate binding component Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae enzyme exists as a complex of at least 3 proteins PIG-A or GPI3, PIG-C or GPI2 and GPI1, in which PIG-A, not PIG-C, is the substrate binding component ?
-
?
additional information Saccharomyces cerevisiae XM37-10c enzyme exists as a complex of at least 3 proteins PIG-A or GPI3, PIG-C or GPI2 and GPI1, in which PIG-A, not PIG-C, is the substrate binding component ?
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Saccharomyces cerevisiae involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Saccharomyces cerevisiae XM37-10c involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
at least 3 components forming the enzyme complex: GPI1, GPI2 or PIG-C, GPI3 or PIG-A
-
Saccharomyces cerevisiae XM37-10c
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme exists as a complex of at least 3 proteins PIG-A or GPI3, PIG-C or GPI2 and GPI1, in which PIG-A, not PIG-C, is the substrate binding component Saccharomyces cerevisiae ?
-
?
additional information enzyme exists as a complex of at least 3 proteins PIG-A or GPI3, PIG-C or GPI2 and GPI1, in which PIG-A, not PIG-C, is the substrate binding component Saccharomyces cerevisiae XM37-10c ?
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Saccharomyces cerevisiae UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Saccharomyces cerevisiae UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Saccharomyces cerevisiae XM37-10c UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Saccharomyces cerevisiae XM37-10c UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
?