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Literature summary for 2.4.1.196 extracted from
- Enzymatic studies on the reversible synthesis of nicotinic acid-N-glucoside in heterotrophic parsley cell suspension cultures (1988), Z. Naturforsch. C, 43c, 835-842.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Petroselinum crispum |  |
| Zn2+ | - |
Petroselinum crispum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
nicotinic acid | - |
Petroselinum crispum | |
| 0.22 | - |
nicotinic acid | - |
Petroselinum crispum | |
| 1.2 | - |
UDPglucose | - |
Petroselinum crispum | |
| 4.3 | - |
UDPglucose | - |
Petroselinum crispum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Petroselinum crispum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for divalent cations | Petroselinum crispum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + nicotinate | Petroselinum crispum | the reversible glucosyltransferase reaction provides free nicotinate for the synthesis of NAD+ and NADP+ in pyridine nucleotide cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petroselinum crispum | - |
Hoffm., parsley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Petroselinum crispum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | heterotrophic | Petroselinum crispum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Petroselinum crispum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + catechol | - |
Petroselinum crispum | ? | - |
? | |
| UDP-glucose + nicotinate | high specificity for UDPglucose as glucosyl donor | Petroselinum crispum | UDP + N-glucosylnicotinate | - |
r | |
| UDP-glucose + nicotinic acid methyl ester | - |
Petroselinum crispum | UDP + N-glucosylnicotinic acid methyl ester | - |
? | |
| UDP-glucose + vanillic acid | - |
Petroselinum crispum | ? | - |
? | |
| UDP-glucose + vanillin | - |
Petroselinum crispum | ? | - |
? | |
| UDPglucose + nicotinate | the reversible glucosyltransferase reaction provides free nicotinate for the synthesis of NAD+ and NADP+ in pyridine nucleotide cycle | Petroselinum crispum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46000, SDS-PAGE | Petroselinum crispum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Petroselinum crispum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.2 | - |
Petroselinum crispum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | at pH 5 nearly inactive, at pH 11 about 50% of activity maximum | Petroselinum crispum |
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