Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | about 110% activity at 10 mM | Parageobacillus thermoglucosidasius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | less than 20% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Ba2+ | about 55% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Cd2+ | about 35% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Co2+ | about 70% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Cu2+ | about 20% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Fe2+ | about 58% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Fe3+ | about 28% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Hg2+ | about 23% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
K+ | about 85% residual activity at 1 mM | Parageobacillus thermoglucosidasius | |
Mn2+ | about 40% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Na+ | about 90% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
Pb2+ | about 42% residual activity at 10 mM | Parageobacillus thermoglucosidasius | |
SDS | complete inhibition at 1 mM | Parageobacillus thermoglucosidasius | |
Zn2+ | about 10% residual activity at 10 mM | Parageobacillus thermoglucosidasius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 110% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Cd2+ | about 110% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Cu2+ | about 108% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Li+ | about 118% activity at 10 mM | Parageobacillus thermoglucosidasius | |
Mg2+ | about 130% activity at 10 mM | Parageobacillus thermoglucosidasius | |
Mn2+ | about 115% activity at 1 mM | Parageobacillus thermoglucosidasius | |
Ni2+ | about 140% activity at 10 mM | Parageobacillus thermoglucosidasius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | - |
- |
- |
Parageobacillus thermoglucosidasius CHB1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration | Parageobacillus thermoglucosidasius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + glycosyl acceptor | - |
Parageobacillus thermoglucosidasius | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
maltodextrin + glycosyl acceptor | - |
Parageobacillus thermoglucosidasius CHB1 | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
potato starch + glycosyl acceptor | - |
Parageobacillus thermoglucosidasius | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
potato starch + glycosyl acceptor | - |
Parageobacillus thermoglucosidasius CHB1 | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | 60.3% conversion rate with 3% (w/v) soluble starch | Parageobacillus thermoglucosidasius | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
soluble starch + glycosyl acceptor | 60.3% conversion rate with 3% (w/v) soluble starch | Parageobacillus thermoglucosidasius CHB1 | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Parageobacillus thermoglucosidasius |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Parageobacillus thermoglucosidasius |
cyclodextrin glycosyltransferase | - |
Parageobacillus thermoglucosidasius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Parageobacillus thermoglucosidasius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | the enzyme maintains more than 50% cyclization activity between 50 and 80°C | Parageobacillus thermoglucosidasius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at incubation temperatures below 50°C, the enzyme maintains residual activity higher than 97% after 2 h. The residual activity of the enzyme decreases rapidly at temperatures higher than 50°C but remains stable at a lower level of activity | Parageobacillus thermoglucosidasius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Parageobacillus thermoglucosidasius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | after incubation at various pH values within a wide range (pH 5.5-9.5) for 2 h, the enzyme shows higher than 90% residual activity | Parageobacillus thermoglucosidasius |