Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenibacillus barengoltzii |
Protein Variants | Comment | Organism |
---|---|---|
A137V | the mutation produces a perturbation in the catalytic site of the enzyme which correlates with a 10fold reduction in its catalytic efficiency. Moreover, this mutant shows increased production of maltooligosaccharides with a high degree of polymerization, mostly maltopentaose to maltoheptaose | Paenibacillus barengoltzii |
A144V | the mutant shows slightly reduced activity compared to the wild type enzyme | Paenibacillus barengoltzii |
L280A | the mutant displays an about 30% reduction in its catalytic efficiency as compared to the wild type enzyme | Paenibacillus barengoltzii |
M329I | the mutant shows slightly reduced activity compared to the wild type enzyme | Paenibacillus barengoltzii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus barengoltzii | - |
- |
- |
Paenibacillus barengoltzii DF 9 R | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose 4B column chromatography | Paenibacillus barengoltzii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cassava starch + D-glucose | the native and recombinant enzymes show 48.8% and 47.8% conversion, respectively | Paenibacillus barengoltzii | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin + maltooligosaccharides | - |
? | |
cassava starch + D-glucose | the native and recombinant enzymes show 48.8% and 47.8% conversion, respectively | Paenibacillus barengoltzii DF 9 R | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin + maltooligosaccharides | - |
? | |
soluble potato starch + glycosyl acceptor | the native and recombinant enzymes show 45.2% and 43.2% conversion, respectively | Paenibacillus barengoltzii | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? | |
soluble potato starch + glycosyl acceptor | the native and recombinant enzymes show 45.2% and 43.2% conversion, respectively | Paenibacillus barengoltzii DF 9 R | beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 74000, SDS-PAGE | Paenibacillus barengoltzii |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Paenibacillus barengoltzii |
cyclodextrin glycosyltransferase | - |
Paenibacillus barengoltzii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the enzyme is inactivated by treatment at 100°C for 5 min | Paenibacillus barengoltzii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.8 | - |
soluble potato starch | mutant enzyme A137V, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii | |
183.9 | - |
soluble potato starch | mutant enzyme L280A, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii | |
231.6 | - |
soluble potato starch | mutant enzyme M329I, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii | |
238.2 | - |
soluble potato starch | mutant enzyme A144V, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii | |
245.3 | - |
soluble potato starch | recombinant wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii | |
250.4 | - |
soluble potato starch | native wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C | Paenibacillus barengoltzii |