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Literature summary for 2.4.1.19 extracted from

  • Castillo, J.; Caminata Landriel, S.; Sanchez Costa, M.; Taboga, O.A.; Berenguer, J.; Hidalgo, A.; Ferrarotti, S.A.; Costa, H.
    A single mutation in cyclodextrin glycosyltransferase from Paenibacillus barengoltzii changes cyclodextrin and maltooligosaccharides production (2018), Protein Eng. Des. Sel., 31, 399-407 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenibacillus barengoltzii

Protein Variants

Protein Variants Comment Organism
A137V the mutation produces a perturbation in the catalytic site of the enzyme which correlates with a 10fold reduction in its catalytic efficiency. Moreover, this mutant shows increased production of maltooligosaccharides with a high degree of polymerization, mostly maltopentaose to maltoheptaose Paenibacillus barengoltzii
A144V the mutant shows slightly reduced activity compared to the wild type enzyme Paenibacillus barengoltzii
L280A the mutant displays an about 30% reduction in its catalytic efficiency as compared to the wild type enzyme Paenibacillus barengoltzii
M329I the mutant shows slightly reduced activity compared to the wild type enzyme Paenibacillus barengoltzii

Organism

Organism UniProt Comment Textmining
Paenibacillus barengoltzii
-
-
-
Paenibacillus barengoltzii DF 9 R
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose 4B column chromatography Paenibacillus barengoltzii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cassava starch + D-glucose the native and recombinant enzymes show 48.8% and 47.8% conversion, respectively Paenibacillus barengoltzii alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin + maltooligosaccharides
-
?
cassava starch + D-glucose the native and recombinant enzymes show 48.8% and 47.8% conversion, respectively Paenibacillus barengoltzii DF 9 R alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin + maltooligosaccharides
-
?
soluble potato starch + glycosyl acceptor the native and recombinant enzymes show 45.2% and 43.2% conversion, respectively Paenibacillus barengoltzii beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin
-
?
soluble potato starch + glycosyl acceptor the native and recombinant enzymes show 45.2% and 43.2% conversion, respectively Paenibacillus barengoltzii DF 9 R beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin
-
?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Paenibacillus barengoltzii

Synonyms

Synonyms Comment Organism
CGTase
-
Paenibacillus barengoltzii
cyclodextrin glycosyltransferase
-
Paenibacillus barengoltzii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the enzyme is inactivated by treatment at 100°C for 5 min Paenibacillus barengoltzii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.8
-
soluble potato starch mutant enzyme A137V, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii
183.9
-
soluble potato starch mutant enzyme L280A, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii
231.6
-
soluble potato starch mutant enzyme M329I, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii
238.2
-
soluble potato starch mutant enzyme A144V, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii
245.3
-
soluble potato starch recombinant wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii
250.4
-
soluble potato starch native wild type enzyme, beta-cyclizing activity, at pH 6.4 and 55°C Paenibacillus barengoltzii