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Literature summary for 2.4.1.19 extracted from

  • Lee, Y.; Zhou, Y.; Park, D.; Chang, J.; Choi, Y.
    beta-Cyclodextrin production by the cyclodextrin glucanotransferase from Paenibacillus illinoisensis ZY-08: Cloning, purification, and properties (2013), World J. Microbiol. Biotechnol., 29, 865-873.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenibacillus illinoisensis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 68% residual activity at 10 mM Paenibacillus illinoisensis
Cu2+ 25% residual activity at 10 mM Paenibacillus illinoisensis
Hg2+ complete inhibition at 1 mM Paenibacillus illinoisensis
Mg2+ 89% residual activity at 10 mM Paenibacillus illinoisensis
additional information not inhibited by EDTA Paenibacillus illinoisensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activating effect (113% activity at 10 mM) Paenibacillus illinoisensis
additional information Ni2+ and Na+ ions have weak effects on the enzyme activity Paenibacillus illinoisensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
x * 74000, SDS-PAGE Paenibacillus illinoisensis
74352
-
x * 74352, calculated from amino acid sequence Paenibacillus illinoisensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
soluble starch + glycosyl acceptor Paenibacillus illinoisensis
-
beta-cyclodextrin
-
?
soluble starch + glycosyl acceptor Paenibacillus illinoisensis ZY-08
-
beta-cyclodextrin
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus illinoisensis
-
-
-
Paenibacillus illinoisensis ZY-08
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Paenibacillus illinoisensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin Paenibacillus illinoisensis ?
-
?
additional information the native enzyme does not convert king oyster mushroom powder and enoki mushroom powder, while recombinant enzyme converts king oyster mushroom powder and enoki mushroom powder to beta-cyclodextrin Paenibacillus illinoisensis ZY-08 ?
-
?
soluble starch + glycosyl acceptor
-
Paenibacillus illinoisensis beta-cyclodextrin
-
?
soluble starch + glycosyl acceptor
-
Paenibacillus illinoisensis ZY-08 beta-cyclodextrin
-
?

Subunits

Subunits Comment Organism
? x * 74000, SDS-PAGE Paenibacillus illinoisensis
? x * 74352, calculated from amino acid sequence Paenibacillus illinoisensis

Synonyms

Synonyms Comment Organism
CGTase
-
Paenibacillus illinoisensis
cyclodextrin glucanotransferase
-
Paenibacillus illinoisensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Paenibacillus illinoisensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 about 65% activity at 20°C, around 95-100% activity at 30-50°C, about 50% activity at 60°C Paenibacillus illinoisensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 the enzyme remains fully stable between 20 and 40°C for 30 min, while at 50°C activity decreases to about 65% and is lost at 60°C Paenibacillus illinoisensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Paenibacillus illinoisensis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 more than 60% activity between pH 5.0 and 9.0 Paenibacillus illinoisensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the enzyme is stable from pH 6.0 to 9.0 for 30 min with a gradual loss of activity at higher and lower pH values in the range of 6.0-9.0 Paenibacillus illinoisensis