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Literature summary for 2.4.1.19 extracted from
- Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans (2013), Acta Crystallogr. Sect. F, 69, 1186-1189.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant Y167H is expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant Y167H, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.05 M Tris-HCl, 0.1 M sodium acetate buffer, 25 mM Na2HPO4, 150 mM NaCl, 10 mM imidazole pH 8.5 | Paenibacillus macerans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y167H | the mutant shows enhanced alpha-cyclodextrin specificity | Paenibacillus macerans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
x * 66000, SDS-PAGE | Paenibacillus macerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | - |
? |  |
| starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | S5ZJ19 | - |
- |
| Paenibacillus macerans 602-1 | S5ZJ19 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant Y167H with ammonium sulfate precipitation and Ni-NTA agarose column chromatography | Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
? | |
| starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Paenibacillus macerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-cyclodextrin glucanotransferase | - |
Paenibacillus macerans |
| CGTase | - |
Paenibacillus macerans |
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Release 2023.1 (January 2023)
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