Protein Variants | Comment | Organism |
---|---|---|
additional information | replacement of B domain by corresponding domain from Thermococcus kodakarensis, Anaerobacter gottschalkii and Pyrococcus furiosus leads to complete loss of catalytic function. Replacement of B domain by corresponding domain from Bacillus stearothermophilus ET1 enzyme leads to a mutant protein with retained activity. The mutant has the wild-type temperature optimum of 60°C with increase in half-life to 57 min. Introduction of mutation F188Y to the mutant results in a half-life of 28 min at 60°C. Bith mutant strains display improved ability to form cyclodextrin and a faster turnover rate | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q5U9V9 | - |
- |
Bacillus sp. (in: Bacteria) G1 | Q5U9V9 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life 22 min for wild-type, 57 min for mutant carrying B domain from Bacillus stearothermophilus ET1 | Bacillus sp. (in: Bacteria) |