Literature summary for 2.4.1.19 extracted from

Kaulpiboon, J.; Prasong, W.; Rimphanitchayakit, V.; Murakami, S.; Aoki, K.; Pongsawasdi, P.
Expression and characterization of a fusion protein-containing cyclodextrin glycosyltransferase from Paenibacillus sp. A11 (2010), J. Basic Microbiol., 50, 427-435.

Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expression of cyclodextrin glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, in Escherichia coli	Paenibacillus sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of cyclodextrin glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, in Escherichia coli. The maximum specific activity for enzyme is 2.7fold higher than that of the non-fusion form, leading to a specific activity of 2268 units/mg protein at a 61% yield. The fusion enzyme is superior than its wild-type counterpart in terms of stability against high temperature and organic solvents. The fusion enzyme catalyzes the synthesis of cyclodextrins in 20% v/v dimethylformamide with a higher product yield of cyclodextrins CD7 and CD8 compared to that of the wild-type enzyme in the same buffer-solvent system	Paenibacillus sp.

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus sp.	-	-	-
Paenibacillus sp. A11	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant fusion protein	Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2268	-	glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, pH 6.0, 40°C	Paenibacillus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
soluble potoato starch	-	Paenibacillus sp.	cyclodextrin	-	?
soluble potoato starch	-	Paenibacillus sp. A11	cyclodextrin	-	?

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Release 2023.1 (January 2023)