Cloned (Comment) | Organism |
---|---|
phylogenetic analysis | Klebsiella pneumoniae |
phylogenetic analysis | Thermoanaerobacter sp. |
phylogenetic analysis | Bacillus sp. (in: Bacteria) |
phylogenetic analysis, expression in Bacillus subtilis strain DB104A | Thermoanaerobacterium thermosulfurigenes |
phylogenetic analysis, expression in Bacillus subtilis strain NA-1 | Geobacillus stearothermophilus |
phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) | Thermococcus sp. |
phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) | Anaerobranca gottschalkii |
phylogenetic analysis, expression of the enzyme from strain BC8 in Escherichia coli strain JM103, and of the enzyme from strain BC251 in Bacillus subtilis strain DB104A | Niallia circulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics | Klebsiella pneumoniae | |
additional information | - |
additional information | reaction kinetics | Niallia circulans | |
additional information | - |
additional information | reaction kinetics | Thermoanaerobacterium thermosulfurigenes | |
additional information | - |
additional information | reaction kinetics | Thermococcus sp. | |
additional information | - |
additional information | reaction kinetics | Thermoanaerobacter sp. | |
additional information | - |
additional information | reaction kinetics | Geobacillus stearothermophilus | |
additional information | - |
additional information | reaction kinetics | Anaerobranca gottschalkii | |
additional information | - |
additional information | reaction kinetics | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | raise the unfolding temperature of CGTase | Niallia circulans | |
Ca2+ | raise the unfolding temperature of CGTase | Thermoanaerobacterium thermosulfurigenes | |
Ca2+ | raise the unfolding temperature of CGTase | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Niallia circulans | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Thermoanaerobacterium thermosulfurigenes | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Thermococcus sp. | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Thermoanaerobacter sp. | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Geobacillus stearothermophilus | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Anaerobranca gottschalkii | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Bacillus sp. (in: Bacteria) | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Thermococcus sp. B1001 | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Geobacillus stearothermophilus NO2 | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Thermoanaerobacterium thermosulfurigenes EM1 | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? | |
additional information | Klebsiella pneumoniae M5a1 | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anaerobranca gottschalkii | - |
- |
- |
Bacillus sp. (in: Bacteria) | P05618 | alkalophilic, strain A2-5a | - |
Geobacillus stearothermophilus | P31797 | - |
- |
Geobacillus stearothermophilus NO2 | P31797 | - |
- |
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae M5a1 | - |
- |
- |
Niallia circulans | - |
strains BC8 and BC251 | - |
Thermoanaerobacter sp. | - |
strain ATCC 53627 | - |
Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Thermoanaerobacterium thermosulfurigenes EM1 | - |
- |
- |
Thermococcus sp. | - |
- |
- |
Thermococcus sp. B1001 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies | Thermococcus sp. |
Renatured (Comment) | Organism |
---|---|
recombinant CGTase from inclusion bodies by solubilization in 6 M urea, refolded by dialysis and heated to 80°C for 20 min | Thermococcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Klebsiella pneumoniae | - |
commercial preparation | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
beta-cyclization | Klebsiella pneumoniae |
23 | - |
beta-cyclization | Thermococcus sp. |
28 | - |
beta-cyclization | Anaerobranca gottschalkii |
52 | - |
beta-cyclization by strain BC8 enzyme | Niallia circulans |
94 | - |
beta-cyclization, commercial preparation | Bacillus sp. (in: Bacteria) |
127 | - |
beta-cyclization | Thermoanaerobacter sp. |
255 | - |
beta-cyclization | Thermoanaerobacterium thermosulfurigenes |
255 | - |
beta-cyclization | Geobacillus stearothermophilus |
265 | - |
beta-cyclization by strain BC251 enzyme | Niallia circulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Klebsiella pneumoniae | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Niallia circulans | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Thermoanaerobacterium thermosulfurigenes | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Thermococcus sp. | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Thermoanaerobacter sp. | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Geobacillus stearothermophilus | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Anaerobranca gottschalkii | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview | Klebsiella pneumoniae | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows high also shows hydrolytic activity on potato starch | Thermoanaerobacter sp. | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows high hydrolytic activity on potato starch | Thermoanaerobacterium thermosulfurigenes | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows hydrolytic activity on potato starch | Thermococcus sp. | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows hydrolytic activity on potato starch | Geobacillus stearothermophilus | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows hydrolytic activity on potato starch | Anaerobranca gottschalkii | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme from strain BC251 also shows hydrolytic activity on potato starch. Substrate binding structure of the strain BC251 enzyme, overview | Niallia circulans | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Thermococcus sp. B1001 | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows hydrolytic activity on potato starch | Thermococcus sp. B1001 | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Geobacillus stearothermophilus NO2 | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows hydrolytic activity on potato starch | Geobacillus stearothermophilus NO2 | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Thermoanaerobacterium thermosulfurigenes EM1 | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview. The enzyme also shows high hydrolytic activity on potato starch | Thermoanaerobacterium thermosulfurigenes EM1 | ? | - |
? | |
additional information | CGTases produce a mixture of cyclodextrins from starch consisting of 6 alpha, 7 beta, or 8 gamma glucose units, specificity, overview | Klebsiella pneumoniae M5a1 | ? | - |
? | |
additional information | reaction mechanism, a linear glucan chain binds to the substrate binding subsites of CGTase followed by bond cleavage to yield a covalent glycosyl-enzyme intermediate. The nature of the acceptor molecule in the second step of the reaction, to which the covalently bound oligosaccharide is transferred, determines the enzyme reaction specificity, schematic overview | Klebsiella pneumoniae M5a1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Klebsiella pneumoniae |
CGTase | - |
Niallia circulans |
CGTase | - |
Thermoanaerobacterium thermosulfurigenes |
CGTase | - |
Thermococcus sp. |
CGTase | - |
Thermoanaerobacter sp. |
CGTase | - |
Geobacillus stearothermophilus |
CGTase | - |
Anaerobranca gottschalkii |
CGTase | - |
Bacillus sp. (in: Bacteria) |
cyclodextrin glucanotransferase | - |
Klebsiella pneumoniae |
cyclodextrin glucanotransferase | - |
Niallia circulans |
cyclodextrin glucanotransferase | - |
Thermoanaerobacterium thermosulfurigenes |
cyclodextrin glucanotransferase | - |
Thermococcus sp. |
cyclodextrin glucanotransferase | - |
Thermoanaerobacter sp. |
cyclodextrin glucanotransferase | - |
Geobacillus stearothermophilus |
cyclodextrin glucanotransferase | - |
Anaerobranca gottschalkii |
cyclodextrin glucanotransferase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Klebsiella pneumoniae |
30 | - |
- |
Thermococcus sp. |
30 | - |
assay at | Anaerobranca gottschalkii |
30 | - |
assay at | Bacillus sp. (in: Bacteria) |
30 | - |
strain BC8 enzyme | Niallia circulans |
50 | - |
strain BC251 enzyme | Niallia circulans |
60 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
60 | - |
- |
Thermoanaerobacter sp. |
65 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermococcus sp. |
5.5 | - |
assay at | Bacillus sp. (in: Bacteria) |
6 | - |
- |
Thermoanaerobacterium thermosulfurigenes |
6 | - |
- |
Thermoanaerobacter sp. |
6 | - |
- |
Geobacillus stearothermophilus |
6 | - |
strain BC251 enzyme | Niallia circulans |
6.8 | - |
- |
Klebsiella pneumoniae |
6.8 | - |
strain BC8 enzyme | Niallia circulans |
8.5 | - |
assay at | Anaerobranca gottschalkii |