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Literature summary for 2.4.1.19 extracted from
- Mutations at subsite -3 in cyclodextrin glycosyltransferase from Paenibacillus macerans enhancing alpha-cyclodextrin specificity (2009), Appl. Microbiol. Biotechnol., 83, 483-490.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is used for cyclodextrin production, generation of a modified enzyme with increased alpha-cyclization specificity, overview | Paenibacillus macerans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Paenibacillus macerans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D372K | site-directed mutagenesis, the mutant shows a great shift in substrate specificity towards the production of alpha-cyclodextrin | Paenibacillus macerans |
| D372K/Y89R | site-directed mutagenesis, the mutant enzyme shows a 1.5fold increase in the production of alpha-cyclodextrin, with a concomitant 43% decrease in the production of beta-cyclodextrin compared to the wild-type CGTase | Paenibacillus macerans |
| additional information | generation of a modified enzyme with increased alpha-cyclization specificity by mutations of Asp372 and Tyr89 at subsite -3 in the CGTase, obverview | Paenibacillus macerans |
| Y89D | site-directed mutagenesis, the mutant shows a shift in substrate specificity towards the production of alpha-cyclodextrin | Paenibacillus macerans |
| Y89K | site-directed mutagenesis, the mutant shows a shift in substrate specificity towards the production of alpha-cyclodextrin | Paenibacillus macerans |
| Y89N | site-directed mutagenesis, the mutant shows a shift in substrate specificity towards the production of alpha-cyclodextrin | Paenibacillus macerans |
| Y89R | site-directed mutagenesis, the mutant shows a great shift in substrate specificity towards the production of alpha-cyclodextrin | Paenibacillus macerans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of His-tagged wild-type and mutant enzymes, overview | Paenibacillus macerans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
x * 76000, about, His-tagged wild-type and mutant enzymes, SDS-PAGE | Paenibacillus macerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
strain strain JFB05-01 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Paenibacillus macerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
comparison of alpha-, beta-and gamma-cyclization activity of wild-type and mutant enzymes, overview | Paenibacillus macerans |
| 1.1 | - |
gamma-cyclization, wild-type enzyme | Paenibacillus macerans |
| 32.2 | - |
beta-cyclization, wild-type enzyme | Paenibacillus macerans |
| 190.4 | - |
alpha-cyclization, wild-type enzyme | Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Asp372 and Tyr89 at subsite -3 play important roles in cyclodextrin product specificity of CGTase. Comparison of alpha-, beta- and gamma-cyclization specificity of wild-type and mutant enzymes, overview | Paenibacillus macerans | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, about, His-tagged wild-type and mutant enzymes, SDS-PAGE | Paenibacillus macerans |
| More | homology structure modelling | Paenibacillus macerans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Paenibacillus macerans |
| cyclodextrin glycosyltransferase | - |
Paenibacillus macerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Paenibacillus macerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzymes, the wild-type enzyme shows a half-life of 8.0 h, the mutant enzymes of 6.0-7.9 h, overview | Paenibacillus macerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Paenibacillus macerans |
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