Literature summary for 2.4.1.19 extracted from

Wang, Z.; Qi, Q.; Wang, P.G.
Engineering of cyclodextrin glucanotransferase on the cell surface of Saccharomyces cerevisiae for improved cyclodextrin production (2006), Appl. Environ. Microbiol., 72, 1873-1877.

Cloned(Commentary)

Cloned (Comment)	Organism
the cyclodextrin glucanotransferase gene is cloned into plasmid pYD1, which allows regulated expression, secretion, and detection. The expression of CGTase with a-agglutinin at the N-terminal end on the extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. This surface-anchored CGTase gives the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated products, cyclodextrins, as well as glucose and maltose. The resulting glucose and maltose, which are efficient acceptors in the CGTase coupling reaction, could be consumed by yeast fermentation and thus facilitated cyclodextrin production. On the other hand, ethanol produced by the yeast may form a complex with cyclodextrin and shift the equilibrium in favor of cyclodextrin production. The yeast with immobilized CGTase produces 24.07 mg/ml cyclodextrins when it is incubated in yeast medium supplemented with 4% starch	Niallia circulans

Cloned (Comment)

Organism

the cyclodextrin glucanotransferase gene is cloned into plasmid pYD1, which allows regulated expression, secretion, and detection. The expression of CGTase with a-agglutinin at the N-terminal end on the extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. This surface-anchored CGTase gives the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated products, cyclodextrins, as well as glucose and maltose. The resulting glucose and maltose, which are efficient acceptors in the CGTase coupling reaction, could be consumed by yeast fermentation and thus facilitated cyclodextrin production. On the other hand, ethanol produced by the yeast may form a complex with cyclodextrin and shift the equilibrium in favor of cyclodextrin production. The yeast with immobilized CGTase produces 24.07 mg/ml cyclodextrins when it is incubated in yeast medium supplemented with 4% starch

Niallia circulans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Niallia circulans	-	-	-
Niallia circulans 251	-	-	-

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Release 2023.1 (January 2023)