Any feedback?
Literature summary for 2.4.1.19 extracted from
- Isolation of Paenibacillus illinoisensis that produces cyclodextrin glucanotransferase resistant to organic solvents (2003), Biosci. Biotechnol. Biochem., 67, 334-340.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Benzene | relative activity 106% | Paenibacillus illinoisensis |  |
| Cyclohexane | - |
Niallia circulans | |
| Cyclohexane | - |
Paenibacillus macerans | |
| Cyclohexane | relative activity 102% | Paenibacillus illinoisensis | |
| decane | increases the yield of cyclodextrin | Paenibacillus illinoisensis | |
| ethanol | - |
Niallia circulans | |
| ethanol | - |
Paenibacillus macerans | |
| ethanol | - |
Salimicrobium halophilum | |
| ethanol | increases yield of cyclodextrins, relative activity 104% | Paenibacillus illinoisensis | |
| ethanol | BE101 produces twice as much cyclodextrin | Bacillus sp. (in: Bacteria) | |
| tert-butanol | strain 251, increases the yield of cyclodextrin | Niallia circulans | |
| Toluene | - |
Niallia circulans | |
| Toluene | - |
Paenibacillus macerans | |
| Toluene | - |
Salimicrobium halophilum | |
| Toluene | relative residual activity 102%, relative activity 104% | Paenibacillus illinoisensis | |
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | cyclodextrin production, agriculture chemistry | Paenibacillus illinoisensis |
| nutrition | important enzyme in food industry | Bacillus sp. (in: Bacteria) |
| nutrition | important enzyme in food industry | Paenibacillus macerans |
| nutrition | important enzyme in food industry | Paenibacillus illinoisensis |
| pharmacology | important enzyme in pharmaceutical industry | Bacillus sp. (in: Bacteria) |
| pharmacology | important enzyme in pharmaceutical industry | Paenibacillus macerans |
| pharmacology | important enzyme in pharmaceutical industry | Paenibacillus illinoisensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-propanol | - |
Paenibacillus macerans | |
| chloroform | - |
Paenibacillus macerans | |
| ethanol | BE 101 | Bacillus sp. (in: Bacteria) | |
| ethanol | - |
Paenibacillus macerans | |
| methanol | - |
Paenibacillus macerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
- |
Salimicrobium halophilum |
| 70000 | - |
SDS-PAGE | Paenibacillus illinoisensis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | stable in presence, relative residual activity 82% | Paenibacillus illinoisensis |
| 2-propanol | stable in presence, but not active, relative residual activity 96% | Paenibacillus macerans |
| benzene | relative residual activity 88% | Paenibacillus illinoisensis |
| benzene | relative residual activity 92% | Paenibacillus macerans |
| chloroform | stable in presence | Paenibacillus illinoisensis |
| chloroform | stable in presence, but not active | Paenibacillus macerans |
| cyclohexane | relative residual activity 88% | Paenibacillus illinoisensis |
| cyclohexane | relative residual activity 91% | Paenibacillus macerans |
| decane | decane, nonane, stable in presence | Paenibacillus illinoisensis |
| diphenyl ether | diphenyl ether, 90% activity in presence | Paenibacillus illinoisensis |
| dodecane | stable in presence | Paenibacillus illinoisensis |
| Ethanol | stable in presence, relative residual activity 84% | Paenibacillus illinoisensis |
| Ethanol | stable in presence, but not active, relative residual activity 99% | Paenibacillus macerans |
| hexane | n-hexane, relative residual activity 90% | Paenibacillus macerans |
| hexane | n-hexane, relative residual activity 84% | Paenibacillus illinoisensis |
| Methanol | stable in presence, but not active, relative residual activity 98% | Paenibacillus macerans |
| Methanol | stable in presence, relative residual activity 102% | Paenibacillus illinoisensis |
| nonane | decane, nonane, stable in presence | Paenibacillus illinoisensis |
| octane | n-octane, 90% activity in presence | Paenibacillus illinoisensis |
| p-xylene | p-xylene, relative residual activity 100% | Paenibacillus macerans |
| toluene | relative residual activity 100% | Paenibacillus macerans |
| toluene | relative residual activity 102% | Paenibacillus illinoisensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus ohbensis | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
BE101 | - |
| Bacillus sp. (in: Bacteria) BE101 | - |
BE101 | - |
| Brevibacterium sp. | - |
no. 9605 | - |
| Brevibacterium sp. 9605 | - |
no. 9605 | - |
| Niallia circulans | - |
- |
- |
| Niallia circulans 251 | - |
- |
- |
| Paenibacillus illinoisensis | - |
ST-12K | - |
| Paenibacillus illinoisensis ST-12K | - |
ST-12K | - |
| Paenibacillus macerans | - |
- |
- |
| Priestia megaterium | - |
- |
- |
| Salimicrobium halophilum | - |
- |
- |
| Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenibacillus illinoisensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1200 | - |
- |
Paenibacillus illinoisensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| soluble starch + glycosyl acceptor | - |
Priestia megaterium | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Thermoanaerobacterium thermosulfurigenes | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus ohbensis | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | strain 251, product proportions 9: 82: 9 with addition of tert-butanol, 15: 65: 20 without solvent | r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | ratio 12: 82: 6 | r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis ST-12K | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | ratio 12: 82: 6 | r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) BE101 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Niallia circulans 251 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | strain 251, product proportions 9: 82: 9 with addition of tert-butanol, 15: 65: 20 without solvent | r | |
| soluble starch + glycosyl acceptor | - |
Priestia megaterium | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Niallia circulans | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | beta-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Thermoanaerobacterium thermosulfurigenes | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Salimicrobium halophilum | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus ohbensis | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus illinoisensis ST-12K | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) BE101 | beta-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Niallia circulans 251 | beta-cyclodextrin | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Paenibacillus illinoisensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Paenibacillus illinoisensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 80 | enzyme is stable from 4-45°C, still has 85% activity after 30 min at 60°C, 40% activity after 30 min at 70°C, loses almost all activity after 30 min at 80°C | Paenibacillus illinoisensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Paenibacillus illinoisensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Paenibacillus illinoisensis |
| 5 | 8 | - |
Bacillus sp. (in: Bacteria) |
| 5 | 8 | - |
Paenibacillus macerans |
| 5 | 8 | - |
Paenibacillus illinoisensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Niallia circulans |
| 6 | 10 | - |
Paenibacillus illinoisensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
