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Literature summary for 2.4.1.19 extracted from

  • Lee, S.H.; Kim, Y.W.; Lee, S.; Auh, J.H.; Yoo, S.S.; Kim, T.J.; Kim, J.W.; Kim, S.T.; Rho, H.J.; Choi, J.H.; Kim, Y.B.; Park, K.H.
    Modulation of cyclizing activity and thermostability of cyclodextrin glucanotransferase and its application as an antistaling enzyme (2002), J. Agric. Food Chem., 50, 1411-1415.
    View publication on PubMed

Application

Application Comment Organism
nutrition
-
Bacillus licheniformis
nutrition
-
Paenibacillus macerans
nutrition application as antistaling agent, retards the deterioration process in bread Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
subcloned on pUB140, expressed in Bacillus subtilis LKS88 Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66800
-
wild-type and mutants, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Geobacillus stearothermophilus
-
ET1
-
Geobacillus stearothermophilus
-
NO2
-
Geobacillus stearothermophilus ET1
-
ET1
-
Geobacillus stearothermophilus NO2
-
NO2
-
Klebsiella pneumoniae
-
-
-
Niallia circulans P43379
-
-
Niallia circulans 251 P43379
-
-
Paenibacillus macerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Paenibacillus macerans beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus NO2 beta-cyclodextrin + maltooligosaccharide
-
r
alpha-cyclodextrin + glycosyl acceptor
-
Geobacillus stearothermophilus ET1 beta-cyclodextrin + maltooligosaccharide
-
r
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus maltose + maltotriose + maltotetraose + maltopentaose
-
r
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus NO2 maltose + maltotriose + maltotetraose + maltopentaose
-
r
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus ET1 maltose + maltotriose + maltotetraose + maltopentaose
-
r

Synonyms

Synonyms Comment Organism
CGTse ET1
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Paenibacillus macerans
60
-
mutant strain F191G Geobacillus stearothermophilus
75
-
mutant strain F251I Geobacillus stearothermophilus
80
-
strain ET1 Geobacillus stearothermophilus