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Literature summary for 2.4.1.187 extracted from
- Acceptor substrate selectivity and kinetic mechanism of Bacillus subtilis TagA (2006), Biochemistry, 45, 10895-10904.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-mannopyranosyl]-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | is an I-parabolic, S-parabolic noncompetitive inhibitor with respect ro UDP-ManNAc and an I-linear, S-parabolic noncompetitive inhibitor with respect to the acceptor 3 at nonsaturating levels | Bacillus subtilis |  |
| additional information | activity does not decrease with the addition of EDTA | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Km at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.03 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Km at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.035 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Km at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.193 | - |
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose | apparent Km at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.258 | - |
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose | apparent Km at 600 microM UDP-ManNAc | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity does not improve with the addition of Mg2+ at concentrations up to 30 mM | Bacillus subtilis | |
| additional information | activity does not improve with the addition of Mn2+ at concentrations up to 30 mM | Bacillus subtilis | |
| NaCl | the enzymatic activity is sensitive to NaCl concentration, with the highest activity at 250 mM | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol | the enzyme utilize a sequential mechanism in which both substrates bind prior to product release. The mechanism is not a rapid equilibrium ordered mechanism. | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
| UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
| UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
| UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
| UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
| UDP-N-acetyl-alpha-D-mannosamine + 2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TagA | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 1.97 | 2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.04 | 1.97 | 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.52 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 0.866 | - |
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 6.08 | - |
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 8.617 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 9.183 | - |
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 10.25 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
| 13.92 | - |
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose | apparent Kcat at 600 microM UDP-ManNAc | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
TagA shows optimal acitvity at pH 7.8 in Bis-Tris propane buffer. Tested at room temperatur | Bacillus subtilis |
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