Cloned (Comment) | Organism |
---|---|
gene CGI_10023521, DNA and amino acid sequence determination and analysis, the Pacific oyster encodes three isozymes of glycogenin: CgGN-alpha, CgGN-beta, and CgGN-gamma, genetic structure and sequence comparisons, phylogenetic tree. The isolated three CgGN isoforms contain alternative exon regions. PCR-based quantitative expression analysis. Recombinant expression of FLAG-tagged enzymes in HEK-293T cells, recombinant expression of EGFP-tagged enzyme from pEGFP-N1-CgGN in HeLa cells | Crassostrea gigas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | Crassostrea gigas | - |
UDP + alpha-D-glucosylglycogenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crassostrea gigas | K1R9A7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adductor | CgGN mRNA expression pattern in adductor muscle during different months, overview. CgGN mRNA expression is closely related to glycogen content and CgGS expression | Crassostrea gigas | - |
gonad | CgGN mRNA expression patterns in gonadal tissue during different months, overview | Crassostrea gigas | - |
additional information | seasonal distribution of CgGN transcripts in adductor muscle and gonad, overview | Crassostrea gigas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | - |
Crassostrea gigas | UDP + alpha-D-glucosylglycogenin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60300, about, sequence calculation | Crassostrea gigas |
Synonyms | Comment | Organism |
---|---|---|
CgGN | - |
Crassostrea gigas |
CgGN-alpha | - |
Crassostrea gigas |
CgGN-beta | - |
Crassostrea gigas |
CgGN-gamma | - |
Crassostrea gigas |
glycogenin | - |
Crassostrea gigas |
glycogenin-1 | - |
Crassostrea gigas |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Crassostrea gigas | sequence calculation | - |
5.05 |
General Information | Comment | Organism |
---|---|---|
evolution | the Pacific oyster has three isozymes of glycogenin: CgGN-alpha, CgGN-beta, and CgGN-gamma. Functional motif architecture analysis shows that CgGN is structurally similar to mammalian glycogenin-1. All three CgGN isoforms contain the key domain of glycosyltransferase and the C-terminal domain | Crassostrea gigas |
physiological function | high glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. mRNA expression is closely related to glycogen content and CgGS expression | Crassostrea gigas |