Literature summary for 2.4.1.186 extracted from

Pitcher, J.; Smythe, C.; Cohen, P.
Glycogenin is the priming glucosyltransferase required for the initiation of glycogen biogenesis in rabbit skeletal muscle (1988), Eur. J. Biochem., 176, 391-395.

Search for more literature for 2.4.1.186

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	no inhibition by phosphorylation of catalytic subunit of the glycogen synthase complex; not inhibitory: UDP-pyridoxal, LiBr	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	UDP-glucose	-	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	less effective than Mn2+	Oryctolagus cuniculus
Mn2+	absolutely dependent on divalent cations, Mn2+ can be replaced by Mg2+ with less effectivity	Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	gel filtration, SDS-PAGE, glycogenin glucosyltransferase represents the smaller subunit of glycogen synthase	Oryctolagus cuniculus
124000	-	gel filtration, heterodimeric glycogen synthase complex	Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + glycogenin	Oryctolagus cuniculus	the glycogenin subunit of glycogen synthase, EC 2.4.1.11, catalyzes this reaction, i.e. the enzyme catalyzes its own glucosylation	UDP + glucosylated glycogenin	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	autoglycosylation	Oryctolagus cuniculus
glycoprotein	five molecules of glucose can be transferred to one molecule of glycogenin, which contains already 1 glucose molecule prior to autoglycosylation	Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment)	Organism
separation from glycogen synthase, EC 2.4.1.11, by LiBr	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + glycogenin	autoglycosylation reaction	Oryctolagus cuniculus	UDP + glucosylated glycogenin	glucosylation reaches a plateau, when 5 additional glucose residues have been added to glycogenin	?
UDP-glucose + glycogenin	autoglycosylation reaction	Oryctolagus cuniculus	UDP + glucosylated glycogenin	i.e. primed glycogenin	?
UDP-glucose + glycogenin	i.e. unprimed glycogenin	Oryctolagus cuniculus	UDP + glucosylated glycogenin	glucosylation reaches a plateau, when 5 additional glucose residues have been added to glycogenin	?
UDP-glucose + glycogenin	i.e. unprimed glycogenin	Oryctolagus cuniculus	UDP + glucosylated glycogenin	i.e. primed glycogenin	?
UDP-glucose + glycogenin	the glycogenin subunit of glycogen synthase, EC 2.4.1.11, catalyzes this reaction, i.e. the enzyme catalyzes its own glucosylation	Oryctolagus cuniculus	UDP + glucosylated glycogenin	-	?

Subunits

Subunits	Comment	Organism
More	glycogenin glucosyltransferase, MW 38 kDa, represents the smaller subunit of glycogen synthase, both enzyme form a heterodimeric complex of molar ratio 1:1	Oryctolagus cuniculus

Synonyms

Synonyms	Comment	Organism
glycogenin glycosyltransferase	-	Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Oryctolagus cuniculus

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Release 2023.1 (January 2023)