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Literature summary for 2.4.1.185 extracted from

  • Liu, X.; Lin, C.; Ma, X.; Tan, Y.; Wang, J.; Zeng, M.
    Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis) (2018), Front. Plant Sci., 9, 166 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression in Nicotiana tabacum using the Agrobacterium tumefaciens strain EHA105 transfection method Citrus sinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01516
-
hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.02041
-
naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.02809
-
kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.03678
-
quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.04327
-
diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Citrus sinensis the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo ?
-
-
UDP-glucose + a flavanone Citrus sinensis
-
UDP + a flavanone 7-O-beta-D-glucoside
-
?
UDP-glucose + a flavone Citrus sinensis
-
UDP + a flavone 7-O-beta-D-glucoside
-
?
UDP-glucose + a flavonol Citrus sinensis
-
UDP + a flavonol 7-O-beta-D-glucoside
-
?

Organism

Organism UniProt Comment Textmining
Citrus sinensis
-
cv. Valencia
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit relative expression of CsUGT76F1 in sweet orange peels and pulp during fruit development, overview Citrus sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo Citrus sinensis ?
-
-
additional information in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected Citrus sinensis ?
-
-
UDP-glucose + a flavanone
-
Citrus sinensis UDP + a flavanone 7-O-beta-D-glucoside
-
?
UDP-glucose + a flavone
-
Citrus sinensis UDP + a flavone 7-O-beta-D-glucoside
-
?
UDP-glucose + a flavonol
-
Citrus sinensis UDP + a flavonol 7-O-beta-D-glucoside
-
?
UDP-glucose + diosmetin
-
Citrus sinensis UDP + diosmetin 7-O-beta-D-glucoside
-
?
UDP-glucose + eriodictyol
-
Citrus sinensis UDP + eriodictyol 7-O-beta-D-glucoside
-
?
UDP-glucose + hesperetin
-
Citrus sinensis UDP + hesperetin 7-O-beta-D-glucoside
-
?
UDP-glucose + isosakuranetin
-
Citrus sinensis UDP + isosakuranetin 7-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol the enzyme can form kaempferol 3,7-O-diglucoside Citrus sinensis UDP + kaempferol 7-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol the enzyme can form kaempferol 3,7-O-diglucoside, reaction of EC 2.4.1.91 Citrus sinensis UDP + kaempferol 3-O-beta-D-glucoside
-
?
UDP-glucose + naringenin
-
Citrus sinensis UDP + naringenin 7-O-beta-D-glucoside
-
?
UDP-glucose + quercetin
-
Citrus sinensis UDP + quercetin 7-O-beta-D-glucoside
-
?
UDP-glucose + quercetin reaction of EC 2.4.1.91 Citrus sinensis UDP + quercetin 3-O-beta-D-glucoside
-
?

Synonyms

Synonyms Comment Organism
CsUGT76F1
-
Citrus sinensis
flavonoid glycosyltransferase
-
Citrus sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40 dependent on the substrate Citrus sinensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70 activity range Citrus sinensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.46
-
kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.58
-
quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.71
-
naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
0.77
-
hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
1.6
-
diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 dependent on the substrate Citrus sinensis

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity range Citrus sinensis

General Information

General Information Comment Organism
malfunction overexpression of the gene significantly increases the accumulations of quercetin 7-O-rhamnoside, quercetin 7-O-glucoside, and kaempferol 7-O-glucoside Citrus sinensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.77
-
quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
16.38
-
kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
34.79
-
naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
36.98
-
diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
50.79
-
hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis