Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression in Nicotiana tabacum using the Agrobacterium tumefaciens strain EHA105 transfection method | Citrus sinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01516 | - |
hesperetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.02041 | - |
naringenin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.02809 | - |
kaempferol | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.03678 | - |
quercetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.04327 | - |
diosmetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Citrus sinensis | the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo | ? | - |
- |
|
UDP-glucose + a flavanone | Citrus sinensis | - |
UDP + a flavanone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavone | Citrus sinensis | - |
UDP + a flavone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | Citrus sinensis | - |
UDP + a flavonol 7-O-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus sinensis | - |
cv. Valencia | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | relative expression of CsUGT76F1 in sweet orange peels and pulp during fruit development, overview | Citrus sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo | Citrus sinensis | ? | - |
- |
|
additional information | in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected | Citrus sinensis | ? | - |
- |
|
UDP-glucose + a flavanone | - |
Citrus sinensis | UDP + a flavanone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavone | - |
Citrus sinensis | UDP + a flavone 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + a flavonol | - |
Citrus sinensis | UDP + a flavonol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + diosmetin | - |
Citrus sinensis | UDP + diosmetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + eriodictyol | - |
Citrus sinensis | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + hesperetin | - |
Citrus sinensis | UDP + hesperetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + isosakuranetin | - |
Citrus sinensis | UDP + isosakuranetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol | the enzyme can form kaempferol 3,7-O-diglucoside | Citrus sinensis | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol | the enzyme can form kaempferol 3,7-O-diglucoside, reaction of EC 2.4.1.91 | Citrus sinensis | UDP + kaempferol 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + naringenin | - |
Citrus sinensis | UDP + naringenin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin | - |
Citrus sinensis | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin | reaction of EC 2.4.1.91 | Citrus sinensis | UDP + quercetin 3-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CsUGT76F1 | - |
Citrus sinensis |
flavonoid glycosyltransferase | - |
Citrus sinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | dependent on the substrate | Citrus sinensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | activity range | Citrus sinensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
kaempferol | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.58 | - |
quercetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.71 | - |
naringenin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
0.77 | - |
hesperetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
1.6 | - |
diosmetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | dependent on the substrate | Citrus sinensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range | Citrus sinensis |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of the gene significantly increases the accumulations of quercetin 7-O-rhamnoside, quercetin 7-O-glucoside, and kaempferol 7-O-glucoside | Citrus sinensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.77 | - |
quercetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
16.38 | - |
kaempferol | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
34.79 | - |
naringenin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
36.98 | - |
diosmetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis | |
50.79 | - |
hesperetin | pH 7.5, 30°C, with UDP-glucose, recombinant enzyme | Citrus sinensis |